Effects of arginine vasopressin on cell volume regulation in brain astrocyte in culture

The American Journal of Physiology
D Sarfaraz, C L Fraser

Abstract

Astrocytes initially swell when exposed to hypotonic medium but rapidly return to normal volume by the process of regulatory volume decrease (RVD). The role that arginine vasopressin (AVP) plays in hypotonically mediated RVD in astrocytes is unknown. This study was therefore designed to determine whether AVP might play a role in astrocyte RVD. With the use of 3-O-[3H]methyl-D-glucose to determine water space, AVP treatment resulted in significantly increased 3-O-methyl-D-glucose water space within 30 s of hypotonic exposure (P = 0.0001) and remained significantly elevated above baseline (1. 75 microliter/mg protein) at 5 min (P < 0.021). In contrast, in untreated cells, complete RVD was achieved by 5 min. At 30 s, cell volume with AVP treatment was 37% greater than in cells that received no treatment (2.9 vs. 2.26 microliter/mg protein, respectively; P < 0.006). The rate of cell volume increase (dV/dt) over 30 s was highly significant (0.038 vs. 0.019 microliter. mg protein-1. s-1 in the AVP-treated vs. untreated group; P = 0.0004 by regression analysis). Additionally, the rate of cell volume decrease over the next 4.5 min was also significantly greater with vasopressin treatment (-dV/dt = 0.0027 vs. 0.0013 microliter. mg prote...Continue Reading

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Citations

May 26, 2010·The Journal of Physiology·Stephen K FisherDaniel J Foster
May 7, 2010·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Christian Overgaard-SteensenTroels Ring
May 2, 2008·American Journal of Physiology. Renal Physiology·Juan Carlos AyusAllen Arieff
May 3, 2019·Journal of Neuroendocrinology·Alastair J MacDonaldKate L J Ellacott
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Aug 15, 2000·Journal of Cellular Physiology·K Lange
May 6, 2009·Acta neurochirurgica·Endre SulyokTamás Dóczi
Dec 1, 2021·Expert Opinion on Therapeutic Targets·Ruchira M JhaJ Marc Simard

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