Effects of Arginine Vasopressin on Migration and Respiratory Burst Activity in Human Leukocytes

Open Medicine
Franz J WiedermannWolfgang Lederer

Abstract

Arginine vasopressin can bind to high-affinity vasopressin V1a receptors in human leukocytes. This study aims to investigate the effects of arginine vasopressin on migration and chemotaxis of neutrophils and oxygen free radical release by human leukocytes. Neutrophils and monocytes were obtained from peripheral blood samples of ten healthy volunteers. Leukocyte migration was microscopically assessed in a modified 48-blind well microchemotaxis chamber, and respiratory burst activity was estimated using 2',7'-dichlorofluorescin diacetate in descending concentrations of arginine vasopressin. Arginine vasopressin stimulates migration of monocytes and neutrophils depending on concentration and on interaction with other chemoattractants. The strongest chemotactic responses of monocytes to arginine vasopressin were observed in the micro and nanomolar range and in the nanomolar range for neutrophils (p<0.001). Pre-incubation of leukocytes with arginine vasopressin decreased migration of leukocytes in a dose-dependent manner. Arginine vasopressin did not stimulate release of oxygen free radicals by neutrophils. Arginine vasopressin stimulates in a dose-dependent manner the migration of monocytes and neutrophils. However, pre-incubation ...Continue Reading

References

Jan 1, 1977·International Archives of Allergy and Applied Immunology·H U KellerH Cottier
Nov 25, 1992·Journal of Immunological Methods·A R RosenkranzG J Zlabinger
Jan 1, 1988·American Journal of Nephrology·C C DohertyS G Massry
Jan 1, 1987·Annals of the New York Academy of Sciences·R Peck
Aug 1, 1981·The Journal of Clinical Investigation·L H BlockW Vetter
Jul 1, 1983·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E Fernandez-RepolletA Schwartz
May 6, 1998·British Journal of Rheumatology·I C Chikanza, A S Grossman
Jul 11, 2000·Journal of Molecular and Cellular Cardiology·S Dunzendorfer, C J Wiedermann
May 15, 2002·Critical Care Medicine·Franz J WiedermannWolfgang Schobersberger
Oct 16, 2002·American Journal of Respiratory and Critical Care Medicine·E Rand SutherlandMonica Kraft
Feb 18, 2004·Bulletin of Experimental Biology and Medicine·I I KhegaiL N Ivanova
Nov 30, 2004·Critical Care : the Official Journal of the Critical Care Forum·Grietje Ch BeckBenito A Yard
Oct 31, 2007·The Journal of Experimental Medicine·Cécilia ChassinAlain Vandewalle
Feb 25, 2009·Annals of the New York Academy of Sciences·Istvan BercziKalman Kovacs
Sep 11, 2009·The Journal of Trauma·Paul J McMahon, Kenneth G Proctor
Jul 1, 1946·Physiological Reviews·M McCUTCHEON
Mar 6, 2013·Journal of Cellular Physiology·Esther GiraldoEduardo Ortega

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Citations

Jan 23, 2021·Biomedicines·Samantha SparapaniChiara Gamberi

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Methods Mentioned

BETA
density gradient centrifugation

Software Mentioned

GraphPad Prism
SPSS

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