Effects of ascorbic acid on collagen mRNA levels in short term chondrocyte cultures

Connective Tissue Research
L J Sandell, J C Daniel

Abstract

Chondrocytes isolated from 16 day chicken embryo sterna and adult (18 month) bovine metacarpalphalangeal joint cartilage were grown in monolayer culture for up to 5 days in the presence and absence of ascorbate (50 micrograms/ml). RNA was isolated from these cultures and the steady-state levels of alpha 1(I), alpha 2(I) and alpha 1(II) mRNAs were assayed using cloned DNA probes encoding the respective procollagen mRNAs. Both ascorbate-treated and control chicken chondrocytes maintained the characteristic morphology and phenotype synthesizing the same levels of type II procollagen mRNA observed for sternal chondrocytes. The chicken chondrocytes, with or without ascorbate, did not synthesize increased levels of alpha 1(I) or alpha 2(I) mRNA. In contrast, when bovine articular chondrocytes were cultured with ascorbate, an increase in type II procollagen mRNA and, more interestingly, an increase in type I procollagen mRNA was observed during the 5 day culture period. Low levels of type I procollagen mRNA were detected in untreated chicken and bovine cultured chondrocytes and chicken chondrocytes isolated from sterna. These experiments suggest that when cultured in the presence of ascorbate under the conditions examined, chicken emb...Continue Reading

References

Jul 1, 1972·Experimental Cell Research·D L LaymanE J Miller
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S MuradS R Pinnell
Dec 1, 1984·The Journal of Cell Biology·J C DanielK E Kuettner
Feb 1, 1983·Molecular and Cellular Biology·L B Rowe, R I Schwarz

❮ Previous
Next ❯

Citations

Sep 1, 1992·Metabolic Brain Disease·I LaszkiewiczG Konat
Feb 20, 2002·Matrix Biology : Journal of the International Society for Matrix Biology·Amy G ClarkVirginia B Kraus
Jul 1, 1997·Annals of the Rheumatic Diseases·T McAlindon, D T Felson
Jan 29, 2016·Annals of Biomedical Engineering·Cushla M McGoverinNancy Pleshko
Jan 1, 1992·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·H R LeipoldG Lust
Jan 1, 1988·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·C A McDevittL Sokoloff
Jun 17, 2008·Osteoarthritis and Cartilage·R AndriamanalijaonaK Boumediene
Apr 1, 1992·Analytical Biochemistry·M E AdamsL J Sandell
Nov 20, 1998·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·H ChanskyL J Sandell
Dec 12, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·A R ShikhmanM K Lotz
Oct 15, 1998·The Journal of Nutrition·D M FracalossiO T Oftedal

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.