Effects of Astragalus injection on proliferation of basal-like breast cancer cell line MDA-MB-468

Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine
Mei-na Ye, Hong-feng Chen

Abstract

To investigate the effects of Astragalus injection (AI) on basal-like breast cancer cell line MDA-MB-468 and murine bone marrow stromal stem cells (mMSCs). MDA-MB-468 cells and primary cultured mMSCs were treated by different concentrations of AI, and with untreated MDA-MB-468 cells as blank control. The morphology of cells was observed by phase-contrast inverted microscope and transmission electron microscopy. Cytotoxic effects of AI on MDA-MB-468 cells and mMSCs were evaluated by cell counting kit-8 (CCK-8) assay. Cell cycle and apoptosis of MDA-MB-468 cells induced by AI were measured by flow cytometry. Lactate dehydrogenase (LDH) activity in supernatants was measured by enzymatic colorimetric method. The expressions of epidermal growth factor receptor (EGFR) and p53 protein in MDA-MB-468 cells were evaluated by streptavidin-biotin-peroxidase complex method. A time-dependent cytotoxic effect of 1 g/ml AI was observed in MDA-MB-468 cells. 1 g/ml AI also had cytotoxic effect on mMSCs, but its effect was not better than cisplatin. 0.1 g/ml AI could promote the proliferation of mMSCs. Different concentrations of AI could all induce the apoptosis of MDA-MB-468 cells. There was no significant difference in LDH activity in the supe...Continue Reading

References

Jun 28, 2003·Proceedings of the National Academy of Sciences of the United States of America·Therese SorlieDavid Botstein
Aug 26, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Torsten O NielsenCharles M Perou
Mar 23, 2005·Cancer Research·François BertucciDaniel Birnbaum

❮ Previous
Next ❯

Citations

Dec 18, 2009·Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine·Ying Deng, Hong-feng Chen
Dec 14, 2011·Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine·Mei-na YeMing-juan Liao
Nov 13, 2014·Evidence-based Complementary and Alternative Medicine : ECAM·Ming-Juan LiaoHong-Feng Chen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis