Effects of Astragalus polysaccharides (APS) on the expression of immune response genes in head kidney, gill and spleen of the common carp, Cyprinus carpio L

International Immunopharmacology
Chuntao YuanXiaodong Han

Abstract

For fish immune defences, cytokines and anti-microbial peptides (lysozyme) in circulating system play important roles. In the present study, the effects of Astragalus polysaccharides (APS) injection on gene expression of interleukin 1beta (IL-1beta), tumor necrosis factoralpha (TNF-alpha) and lysozyme-C in the head kidney, gill and spleen of common carp were determined using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). After injection of APS, IL-1beta mRNA level increased in a dose-dependent manner in the head kidney, while no significant changes were found in the gill and spleen. High dose of APS up regulated TNF-alpha transcription in the gill and spleen, while TNF-alpha mRNA level decreased significantly in the head kidney of low dose of APS. Lysozyme-C mRNA levels were up regulated in the gill of low dose of APS and spleen of middle dose of APS. No effect of the APS on lysozyme-C expression was observed in head kidney. These results constitute a first step toward the understanding of APS effect on cytokines and immune-related gene expression in different organs of common carp.

References

Apr 15, 1988·European Journal of Biochemistry·B GrindeP Jollès
May 1, 1994·Veterinary Immunology and Immunopathology·A J Ainsworth
Jul 1, 1993·Developmental and Comparative Immunology·Rolf E Engstad, Børre Robertsen
Jan 23, 1999·Immunological Reviews·C J SecombesG Kemp
Aug 5, 2000·Biological & Pharmaceutical Bulletin·E BedirI A Khan
Feb 13, 2001·Developmental and Comparative Immunology·M Y EngelsmaB M Verburg-van Kemenade
Oct 17, 2001·Developmental and Comparative Immunology·C J SecombesJ Zou
Jul 14, 2004·Biochemical and Biophysical Research Communications·Bao-Mei ShaoXiao-Ming Gao
Sep 12, 2006·Journal of Ethnopharmacology·Guan-Cheng HuangChing-Chiung Wang
Sep 19, 2006·International Journal of Biological Macromolecules·Vaclav VetvickaJean-Claude Yvin
Jan 16, 2007·Cancer Letters·William C S Cho, Kwok N Leung

Citations

Mar 12, 2009·Fish Physiology and Biochemistry·Jeney GalinaZ Jeney
Dec 12, 2013·International Journal of Biological Macromolecules·Mingliang JinPeng Shang
Dec 5, 2009·World Journal of Gastroenterology : WJG·Shuang-Suo DangEn-Qi Liu
Feb 6, 2009·World Journal of Gastroenterology : WJG·Di NaHui-Mian Xu
Mar 9, 2013·Fish & Shellfish Immunology·S PicchiettiA M Fausto
Apr 4, 2015·Carbohydrate Polymers·Li-Chan YangWen-Chuan Lin
Jun 18, 2019·Advances in Nutrition·Jianmin WuXi Ma
Apr 9, 2020·Frontiers in Pharmacology·Yijun ZhengYongqi Liu

Related Concepts

Related Feeds

Autoimmune Polyendocrine Syndromes

This feed focuses on a rare genetic condition called Autoimmune Polyendocrine Syndromes, which are characterized by autoantibodies against multiple endocrine organs. This can lead to Type I Diabetes.

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Autoimmune Polyendocrinopathies

Autoimmune polyendocrinopathies, also called polyglandular autoimmune syndromes (PGASs), or polyendocrine autoimmune syndromes(PASs), are a heterogeneous group of rare diseases characterized by autoimmune activity against more than one endocrine organ, although non-endocrine organs can be affected. Discover the latest research on autoimmune polyendocrinopathies here.