PMID: 9178188May 1, 1997Paper

Effects of ATP on phosphoinositide hydrolysis and prostaglandin E2 generation in rabbit astrocytes

The Journal of Pharmacy and Pharmacology
H IshimotoH Nakanishi

Abstract

Extracellular ATP secreted from stimulated nerves plays a role in neurotransmission. This study examined the effects of extracellular ATP on phospholipase A2 and C signalling pathways in rabbit astrocytes. ATP caused prostaglandin E2 (PGE2) generation and phosphoinositide hydrolysis in a time- and concentration-dependent manner. A P2y purinoceptor-selective agonist, 2-methylthio-ATP also caused phosphoinositide hydrolysis, but not PGE2 generation. A P2x purinoceptor-selective agonist, alpha, beta-methylene-ATP did not cause either phosphoinositide hydrolysis or PGE2 generation. Although pertussis toxin had no effect on 2-methylthio-ATP-induced phosphoinositide hydrolysis, it markedly decreased ATP-induced PGE2 generation, with significant inhibition of phosphoinositide hydrolysis. Dexamethasone and indomethacin which potently inhibited ATP-induced PGE2 generation, caused partial inhibition of phosphoinositide hydrolysis, suggesting that pertussis toxin-sensitive component of ATP-induced phospholipase C activation is mediated by cyclo-oxygenase metabolites of arachidonic acid. These results suggest that a stimulation of P2y receptor results in phospholipase C activation in a pertussis toxin-insensitive manner, and that a P2 rece...Continue Reading

References

Feb 19, 1992·Biochimica Et Biophysica Acta·C el-MoatassimJ C Mani
Jan 1, 1985·Clinical Science·G Burnstock, P Sneddon
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·C GuptaR Piddington
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·T HoffmanJ Axelrod
May 1, 1995·Trends in Pharmacological Sciences·C Kennedy, P Leff
Sep 1, 1993·The American Journal of Physiology·G R Dubyak, C el-Moatassim
Jan 29, 1993·Cell·L L LinR J Davis
Jul 1, 1996·Journal of Neurochemistry·H KaihoH Nakanishi

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Citations

Mar 12, 2009·Journal of Molecular Neuroscience : MN·Toshiyuki ChikumaHiroshi Hojo
Mar 14, 2009·Molecular Neurobiology·Alexei VerkhratskyGeoffrey Burnstock
Mar 30, 2010·Acta Physiologica·G BurnstockA Verkhratsky
Jan 20, 2018·Physiological Reviews·Alexei Verkhratsky, Maiken Nedergaard
Jun 12, 2003·American Journal of Physiology. Renal Physiology·Brett D WelchBellamkonda K Kishore
Oct 22, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·W H PercyC E Wangsness

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