Effects of Atrial Natriuretic Peptide on p53 and Estrogen Receptor in Breast Cancer Cells

BioResearch Open Access
Katie AleckSumi Dinda

Abstract

The atrial natriuretic peptide (ANP) hormone is secreted by cardiac atrial myocytes and acts to regulate blood pressure homeostasis in humans. Previous research indicates ANP treatment significantly decreases the proliferation of human prostate cancer cells, pancreatic adenocarcinoma, and breast cancer cells. Minimal studies have been conducted with regard to ANP regulating tumor suppressor genes and steroid hormone receptors in breast cancer cells. Our study analyzed the effects of ANP in combination with 17β-estradiol (E2) and antiestrogen treatments on p53 and ERα levels in T-47D breast cancer cells. Preliminary studies through Western blot analysis showed that ANP treatment decreases p53 and ERα expression levels in a concentration-dependent (10-100 nM) manner. Treatment with ANP alone, at a 100 nM concentration, causes a decrease of p53 and ERα expression compared with Cs (control stripped), but with E2 and antiestrogen combinations, expression of both protein levels decreased compared with treatments without ANP. Combined treatment with E2, an estrogen antagonist, and ANP decreased cellular proliferation compared with treatments without ANP, except in the case of raloxifene (RAL). Our studies indicate that ANP has potenti...Continue Reading

References

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Citations

Nov 19, 2020·Breast Cancer : Targets and Therapy·Andrea Luque-BolivarMilena Rondón-Lagos
Jun 7, 2020·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Bárbara CostaNuno Vale

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Methods Mentioned

BETA
protein assay
electrophoresis
confocal microscopy

Software Mentioned

Image Studio Lite
NIS elements
SPSS

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