Effects of autologous, cross-linked erythrocytes on isolated hypoperfused rabbit heart dynamics

Pharmacology
Maria FrosiniMassimo Valoti

Abstract

The present study was aimed at assessing the effects of either red blood cells (RBC) or RBC cross-linked with the bifunctional dimethyl suberimidate reagent (C-RBC) on contractile force (CFo), heart rate (HR) and coronary flow (CF) of the isolated rabbit heart hypoperfused with RBC suspensions under 30 mm Hg constant pressure. RBC or C-RBC caused a rapid and marked reduction of CF, CFo and HR. In RBC-treated hearts, however, reperfusion with Tyrode solution partially restored the initial myocardial parameters, while in C-RBC-treated hearts a rapid impairment of diastolic relaxation with a subsequent, steady and increasing heart contracture was observed. Histological analysis showed that in C-RBC-perfused hearts either capillaries or precapillary arterioles were occluded by C-RBC in spite of extensive washings with Tyrode solution. These findings indicate that C-RBC impair coronary circulation markedly and irreversibly.

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Citations

Feb 19, 2016·Clinical Hemorheology and Microcirculation·Marijke GrauFrank Suhr

Related Concepts

Adenosine Triphosphate, Chromium Ammonium Salt
Coronary Circulation
Cross-Linking Reagents
Dimethyl Suberimidate
Erythrocytes
Heart
Pulse Rate
Myocardial Contraction
Perfusion
Structure of Arteriole

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