Effects of Autologous Serum Eye Drops on Conjunctival Expression of MUC5AC in Patients With Ocular Surface Disorders

Cornea
José Santiago López-GarcíaTatiana Suárez-Cortés

Abstract

To assess the effects of autologous serum eye drops on conjunctival expression of the mucin gene MUC5AC by means of quantitative reverse transcription polymerase chain reaction (RT-qPCR). A prospective and comparative interventional case series study of 38 eyes of 19 patients with different ocular surface disorders was performed before and 6 weeks after the treatment with autologous serum eye drops. All patients underwent a complete ophthalmic assessment, including evaluation of the tear film, ocular surface exploration, conjunctival impression cytology (IC), and MUC5AC detection by reverse transcription polymerase chain reaction. A total of 34 eyes were studied by IC and MUC5AC quantitative reverse transcription polymerase chain reaction before and after treatment with autologous serum. This treatment improved breakup time, conjunctival squamous metaplasia, goblet cell density, and subjective perception in 76.2%, 70.6%, 55.9% and 73.5% of eyes, respectively. Treatment with autologous serum enhanced conjunctival expression of MUC5AC (P = 0.001), although these differences were not statistically significant if data are analyzed patient by patient (P = 0.09). In 13 of 34 eyes (38.2%), we found increased expression of MUC5AC; in 1...Continue Reading

Associated Clinical Trials

Jan 3, 2020·Wan Haslina Wan Abdul Halim

References

Oct 28, 1997·Fertility and Sterility·L LapenséeG Bleau
Aug 6, 1999·The British Journal of Ophthalmology·K TsubotaS Shimmura
Nov 1, 2002·Cornea·Jose Manuel Benítez del CastilloJulian García Sánchez
Dec 28, 2002·Cornea·Marcia M JumblattJames E Jumblatt
May 27, 2004·Investigative Ophthalmology & Visual Science·Leonard P K AngRobert M Lavker
Jun 5, 2004·Ophthalmology·Yukihiro MatsumotoKazuo Tsubota
Oct 19, 2004·The British Journal of Ophthalmology·G GeerlingD Hartwig
Jan 31, 2007·Archivos de la Sociedad Española de Oftalmología·J S López-GarcíaJ Martínez-Garchitorena
Aug 7, 2007·Ophthalmology·J S López-GarcíaJ Murube
Oct 5, 2010·Ophthalmology·Jose Santiago López-GarcíaJuan Murube del Castillo
Jul 31, 2013·Acta Ophthalmologica·José S López-GarcíaMaria T Méndez

❮ Previous
Next ❯

Citations

Mar 31, 2017·PloS One·Iñaki Rodriguez-AgirretxeArantxa Acera
Nov 29, 2017·Biomarkers in Medicine·Suzanne Hagan
Nov 28, 2018·Investigative Ophthalmology & Visual Science·Akihiro Higuchi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.