Effects of autophagy regulation of tumor‑associated macrophages on radiosensitivity of colorectal cancer cells

Molecular Medicine Reports
Le-Ning ShaoJian-Ping Cao

Abstract

Tumor‑associated macrophages (TAMs), a major component of the tumor microenvironment, are crucial to the processes of tumor growth, infiltration and metastasis, and contribute to drug resistance. The importance of TAMs in radiation resistance of colorectal cancer remains unclear. To investigate the effects of autophagy regulation of TAMs on the radiosensitivity of colorectal cancer cells, the current study induced TAM formation from THP‑1 monocyte cells. Sequential treatment of THP‑1 cells with PMA for 72 h and human recombinant interleukin‑4 for 24 h was used to stimulate THP‑1 differentiation to TAMs. Expression of the cell surface markers CD68, CD204 and CD206, and changes to cell morphology were used to confirm successful differentiation. The TAMs were stimulated to promote or inhibit autophagy during co‑culture with LoVo colorectal adenocarcinoma cells. The cells were irradiated, with subsequent measurement of LoVo colony formation and apoptosis. Additionally, the expression of p53, Bcl‑2, survivin and Smac proteins was assessed by western blotting. Monodansylcadaverin staining was used to analyze the presence of autophagic vacuoles in TAM, and western blot analysis was used to assess the expression of Beclin‑1, LC3B I and...Continue Reading

References

Mar 1, 1995·World Journal of Surgery·M Gutman, I J Fidler
Nov 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Manu KohliLin Zhang
Jun 24, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Robert D StoutJill Suttles
Jan 13, 2006·British Journal of Cancer·L BingleN J Brown
Mar 8, 2006·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Antonio SicaPaola Allavena
Oct 5, 2007·Critical Reviews in Oncology/hematology·Paola AllavenaAlberto Mantovani
Nov 17, 2007·Genes & Development·Noboru Mizushima
Dec 19, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Danielle CanioniLuc Xerri
Sep 3, 2009·Neoplasia : an International Journal for Oncology Research·Ying LinWilliam S Dynan
Oct 14, 2009·Cancer Biology & Therapy·Jayashree Karar, Amit Maity
Oct 31, 2009·International Journal of Cancer. Journal International Du Cancer·Zhaohui HuangXiang Du
Sep 16, 2010·American Journal of Physiology. Endocrinology and Metabolism·Michael SpencerPhilip A Kern
Feb 8, 2011·CA: a Cancer Journal for Clinicians·Ahmedin JemalDavid Forman
Feb 18, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Ravi K AmaravadiEileen White
Jul 27, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jan PanderThorbald van Hall
Nov 5, 2011·International Journal of Cancer. Journal International Du Cancer·Tomonori AkagiSeigo Kitano
Jun 16, 2012·CA: a Cancer Journal for Clinicians·Rebecca SiegelElizabeth Ward
Jun 25, 2015·Nature Reviews. Cancer·Holly E BarkerKevin J Harrington

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Citations

May 4, 2017·Biological Reviews of the Cambridge Philosophical Society·Juan LorenteMatilde E LLeonart
Nov 2, 2019·Cells·Samantha N Devenport, Yatrik M Shah
Dec 5, 2018·Signal Transduction and Targeted Therapy·Yuhui WuWeidong Han
Mar 17, 2021·Journal of Experimental & Clinical Cancer Research : CR·Haoze LiQing Ji

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