Effects of azithromycin, metronidazole, amoxicillin, and metronidazole plus amoxicillin on an in vitro polymicrobial subgingival biofilm model

Antimicrobial Agents and Chemotherapy
Geisla Mary Silva SoaresRicardo Teles

Abstract

Chronic periodontitis is one of the most prevalent human diseases and is caused by dysbiosis of the subgingival microbiota. Treatment involves primarily mechanical disruption of subgingival biofilms and, in certain cases, adjunctive use of systemic antibiotic therapy. In vitro biofilm models have been developed to study antimicrobial agents targeting subgingival species. However, these models accommodate a limited number of taxa, lack reproducibility, and have low throughput. We aimed to develop an in vitro multispecies biofilm model that mimics subgingival plaque, to test antimicrobial agents. Biofilms were cultivated using the Calgary Biofilm Device and were exposed to amoxicillin (AMX), metronidazole (MTZ), azithromycin (AZM), and AMX-MTZ at four different concentrations for 12, 24, or 36 h. Chlorhexidine (CHX) (0.12%) was used as the positive control. The compositions of the biofilms were analyzed by checkerboard DNA-DNA hybridization, and the percent reduction in biofilm metabolic activity was determined using 2,3,5-triphenyltetrazolium chloride and spectrophotometry. Thirty-five of the 40 species used in the inoculum were consistently recovered from the resulting in vitro biofilms. After 36 h of exposure at the 1:27 dilut...Continue Reading

References

Nov 1, 1982·Journal of Clinical Periodontology·S S SocranskyG C Sornberger
Dec 1, 1994·The Journal of Antimicrobial Chemotherapy·M J PavicićJ de Graaff
Apr 1, 1994·Analytical Biochemistry·R P AldertonS Beck
Dec 24, 1997·Journal of Clinical Periodontology·H TenenbaumM Dahan
Mar 12, 1998·Journal of Clinical Periodontology·S S SocranskyR L Kent
Apr 1, 1997·Advances in Dental Research·C H Sissons
Mar 28, 2001·Journal of Dental Research·B GuggenheimS Shapiro
May 24, 2001·Journal of Bacteriology·B J PasterF E Dewhirst
May 16, 2002·Periodontology 2000·Sigmund S Socransky, Anne D Haffajee
Jul 10, 2002·Periodontology 2000·L Jackson BrownThomas P Wall
Oct 20, 2004·Oral Microbiology and Immunology·S S SocranskyJ M Goodson
Feb 5, 2005·Journal of Clinical Periodontology·Ene-Renate PähklaRein Pähkla
Apr 28, 2005·Periodontology 2000·Sigmund S Socransky, Anne D Haffajee
Feb 21, 2007·Journal of Clinical Periodontology·Anne D HaffajeeSigmund S Socransky
Apr 3, 2008·Journal of Periodontology·Flavia R TelesSigmund S Socransky
Nov 4, 2010·The Journal of Microbiology·Shukho KimJungmin Kim
Mar 23, 2011·Journal of Periodontology·Pin-Chuang LaiJohn D Walters
Jul 21, 2012·Journal of Periodontal Research·O A GonzálezC S Miller
Aug 21, 2012·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Dorota KowalczukMałgorzata Miazga-Karska
Sep 1, 2012·Journal of Dental Research·P I EkeR J Genco
Dec 12, 2012·Medicina oral, patología oral y cirugía bucal·María-Carmen SánchezMariano Sanz

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Citations

Nov 26, 2015·Future Microbiology·Rebecca A Gabrilska, Kendra P Rumbaugh
Mar 12, 2016·Journal of Tropical Pediatrics·Mathieu MartyIsabelle Bailleul-Forestier
Sep 20, 2016·Biochemical Pharmacology·Manuel FerrerAndrés Moya
Jul 15, 2017·Journal of Periodontal Research·M Fernandez Y MostajoW Crielaard
Dec 12, 2017·Medical Mycology·Marlene ChevalierIsabelle Prêcheur
Mar 2, 2018·Journal of Dental Research·Y YoshimasuR Nakao
Apr 25, 2019·Biofouling·Stela Lima Farias MirandaBruno Bueno-Silva
Sep 17, 2019·Journal of Medical Microbiology·Jason L BrownGordon Ramage
Oct 3, 2019·Brazilian Oral Research·João PingueiroBruno Bueno-Silva
Nov 24, 2016·Clinical and Experimental Dental Research·Masaaki TakeshitaFusanori Nishimura
Jul 28, 2017·Journal of Oral Microbiology·Hwei Sze OngEric C Reynolds
Jun 20, 2020·Brazilian Dental Journal·Stela Lima Farias de MirandaBruno Bueno-Silva
May 11, 2018·Clinical and Experimental Dental Research·Gabriela Dabija-WolterAnne Isine Bolstad
Nov 12, 2020·Molecular Oral Microbiology·Eleanor I LamontJeffrey S McLean

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