PMID: 9546153Apr 18, 1998Paper

Effects of bacterial host and dichloromethane dehalogenase on the competitiveness of methylotrophic bacteria growing with dichloromethane

Applied and Environmental Microbiology
D GisiS Vuilleumier

Abstract

Methylobacterium sp. strain DM4 and Methylophilus sp. strain DM11 can grow with dichloromethane (DCM) as the sole source of carbon and energy by virtue of homologous glutathione-dependent DCM dehalogenases with markedly different kinetic properties (the kcat values of the enzymes of these strains are 0.6 and 3.3 S-1, respectively, and the Km values are 9 and 59 microM, respectively). These strains, as well as transconjugant bacteria expressing the DCM dehalogenase gene (dcmA) from DM11 or DM4 on a broad-host-range plasmid in the background of dcmA mutant DM4-2cr, were investigated by growing them under growth-limiting conditions and in the presence of an excess of DCM. The maximal growth rates and maximal levels of dehalogenase for chemostat-adapted bacteria were higher than the maximal growth rates and maximal levels of dehalogenase for batch-grown bacteria. The substrate saturation constant of strain DM4 was much lower than the Km of its associated dehalogenase, suggesting that this strain is adapted to scavenge low concentrations of DCM. Strains and transconjugants expressing the DCM dehalogenase from strain DM11, on the other hand, had higher growth rates than bacteria expressing the homologous dehalogenase from strain DM4....Continue Reading

References

Sep 1, 1985·Microbiological Reviews·D K Button
Jan 1, 1983·Annual Review of Microbiology·W Harder, L Dijkhuizen
Dec 1, 1994·Biodegradation·T LeisingerS Vuilleumier
Jan 1, 1993·Antonie van Leeuwenhoek·H W Jannasch, T Egli
Mar 1, 1997·Journal of Bacteriology·S Vuilleumier
Sep 23, 1997·Biochemical and Biophysical Research Communications·S VuilleumierT Leisinger
Mar 1, 1988·Applied and Environmental Microbiology·William E HolbenJames M Tiedje
Jul 1, 1993·Applied and Environmental Microbiology·A J van den WijngaardD B Janssen

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Citations

Aug 5, 2000·Applied and Environmental Microbiology·M F VerceD L Freedman
Mar 10, 2001·Microbiology·S VuilleumierT Leisinger
Jul 17, 2013·Environmental Science and Pollution Research International·Dong-Zhi ChenJian-Meng Chen
Feb 3, 2009·Applied Microbiology and Biotechnology·Shijin J WuJianmeng M Chen
Feb 6, 2020·FEMS Microbiology Reviews·Bruno MaucourtFrançoise Bringel
Aug 23, 2011·Environmental Microbiology·Emilie E L MullerFrançoise Bringel
Mar 1, 2019·Journal of Environmental Management·Carme BarbaNúria Gaju
Nov 18, 1998·Current Opinion in Chemical Biology·S D Copley
May 10, 2003·Chemosphere·Karl Heinz van Pée, Susanne Unversucht

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