Effects of barbiturates on the expression of the inducible nitric oxide synthase in vascular smooth muscle

Journal of Cardiovascular Pharmacology
P KesslerV B Schini-Kerth

Abstract

Certain cytokines stimulate the expression of the inducible nitric oxide synthase (iNOS) in vascular smooth muscle cells (VSMCs) and in many other cell types. The large amounts of nitric oxide (NO) generated by iNOS in the vascular wall contribute to the unrelenting hypotension in septic shock. Because septic patients are often treated with barbiturates, we examined the effect of these anesthetic agents on the expression of iNOS in VSMCs. The induction of iNOS was elicited either in cultured rat aortic SMCs [interleukin-1beta (IL-1beta), 60 U/ml for 24 h] or in endothelium-denuded segments of the rabbit carotid artery [IL-1beta (100 U/ml) for 7 h]. The activity of iNOS was assessed by the accumulation of nitrite in the conditioned medium of cultured VSMCs and by the hyporeactivity of carotid arteries to phenylephrine. Moreover, iNOS protein abundance was determined by Western blot analysis, iNOS messenger RNA (mRNA) by reverse transcription followed by the polymerase chain reaction (PCR), and activation of the transcription factor NF-kappaB by gel electrophoretic mobility-shift analysis of nuclear extracts from VSMCs. The IL-1beta-stimulated increase in nitrite formation, iNOS protein, and mRNA abundance in VSMCs was significan...Continue Reading

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Citations

Oct 14, 2000·Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie·T SakaiJ M Lipton
May 27, 2008·Journal of Anesthesia·Noboru TodaYoshio Hatano
Apr 19, 2007·Clinical and Experimental Pharmacology & Physiology·Shang-Jyh KaoHsing I Chen
Mar 8, 2016·Life Sciences·Wenguo FanHongwen He
Mar 9, 2002·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Emine SipahiFerruh Niyazi Ayoglu
Jun 20, 2017·Magnetic Resonance in Medical Sciences : MRMS : an Official Journal of Japan Society of Magnetic Resonance in Medicine·Fang YuanChunchao Xia
Apr 11, 2001·Journal of Neurosurgical Anesthesiology·D L FeinsteinG Weinberg

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