Effects of BDNF infusion on the regulation of TrkB protein and message in adult rat brain

Experimental Neurology
L FrankJ S Rudge

Abstract

Exposure of embryonic CNS neurons to BDNF in vitro causes down-regulation of TrkB protein and mRNA, and an attenuation of functional responses to acute neurotrophin stimulation. In order to investigate ligand-mediated regulation of TrkB in vivo, we infused BDNF into the midbrain, near the periaquaductal grey-dorsal raphe (PAG-DR), or into the olfactory bulb of adult rats. Midbrain infusion of BDNF produced analgesia that was sustained for the duration of BDNF delivery. Analysis of TrkB receptor levels revealed that at the point when the maximal analgesic effect of BDNF was obtained, there was a concommitant 75% decrease in full-length TrkB protein at the infusion site. After discontinuation of infusion, levels of TrkB recovered toward base line. Interestingly, TrkB protein levels were not accompanied by decreased trkB mRNA levels. To determine if BDNF infusion decreased TrkB protein levels in other brain areas and whether trkB mRNA might be down-regulated in the cell bodies of neurons projecting to the infusion site, we infused BDNF into the olfactory bulb. Following a 12-day infusion of BDNF, TrkB protein levels decreased within the bulb to a similar extent as in the PAG-DR. This decrease in receptor protein, however, was not ...Continue Reading

References

Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·E CastrénD Lindholm
May 1, 1991·Trends in Neurosciences·H Thoenen
May 1, 1994·Trends in Neurosciences·R M LindsayP S DiStefano
Nov 1, 1994·Trends in Neurosciences·O LindvallM Kokaia
Jul 1, 1995·The Journal of Cell Biology·M D EhlersV E Koliatsos
Jun 1, 1993·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A Sorkin, C M Waters
May 1, 1993·Neuron·D M ValenzuelaG D Yancopoulos

❮ Previous
Next ❯

Citations

Oct 31, 2002·The European Journal of Neuroscience·L A KarchewskiV M K Verge
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·A Di PoloA J Aguayo
May 28, 2015·Pharmacology, Biochemistry, and Behavior·Rebeca Vargas Antunes SchunckMirna Bainy Leal
Jan 16, 2013·Neurochemistry International·Boya NugrahaChristoph Gutenbrunner
Oct 24, 2003·The Journal of Comparative Neurology·Alessandro CellerinoKonrad Kohler
Feb 17, 2009·Experimental Eye Research·Elaine C JohnsonJohn C Morrison
Apr 9, 2008·Pharmacology & Therapeutics·Margaret I Davis
Jun 8, 2011·Journal of Neuroscience Research·Cynthia L RennSusan G Dorsey
Dec 11, 2002·Journal of Neurochemistry·Prakasham RumajogeeMarie-Christine Miquel
Feb 17, 2009·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·John GardinerJan Marc
Aug 31, 2002·The Journal of Biological Chemistry·Annakaisa HaapasaloEero Castren
Jan 3, 2012·Rheumatology International·Boya NugrahaChristoph Gutenbrunner
Sep 30, 2020·Stem Cells and Development·Ana Paula Aquistapase DagninoJefferson Braga Silva
Sep 12, 2006·Progress in Retinal and Eye Research·Alan R HarveyQi Cui

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.