Effects of biobanking conditions on six antibiotic substances in human serum assessed by a novel evaluation protocol

Clinical Chemistry and Laboratory Medicine : CCLM
Johannes ZanderMichael Vogeser

Abstract

Although therapeutic drug monitoring (TDM) for antibiotics in critically ill patients is recommended by expert panels, no commercial tests are available for most antibiotics. Therefore, we previously developed a multi-analyte method for the quantification of piperacillin, tazobactam, cefepime, meropenem, ciprofloxacin and linezolid in serum. However, limited stability data were available, and the relevant studies did not address the coefficients of variation of the methods applied, which may be important for verifying the storage dependency of the observed effects. Here we aimed to evaluate the storage effects of antibiotics by applying a novel evaluation protocol. Serum-based test samples were aliquoted and stored at room temperature, 4 °C, -20 °C or -80 °C for up to 180 days. Using an innovative evaluation protocol (considering the coefficient of variation, p-value, and criterion of monotony of observed changes), we assessed whether relevant changes (defined as ≥15% in comparison with baseline) were storage dependent (defined as substantial changes). Storage at -80 °C for up to 180 days did not lead to substantial changes for any analyte. In contrast, storage at -20 °C induced substantial decreases after ≥7 days for piperacil...Continue Reading

References

Jun 28, 1996·Journal of Chromatography. B, Biomedical Applications·V AugeyF Bressolle
Nov 8, 1996·Journal of Chromatography. B, Biomedical Applications·H ElkhaïliF Jehl
Mar 7, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·H ElkhaïliF Jehl
May 8, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·N ChertiF Bressolle
Oct 27, 2001·The Journal of Antimicrobial Chemotherapy·C M TobinA P MacGowan
Dec 17, 2003·Clinical Pharmacokinetics·Craig R RaynerJerome J Schentag
Nov 18, 2005·Biomedical Chromatography : BMC·Lauren M BoakCraig R Rayner
Apr 22, 2006·Therapeutic Drug Monitoring·Ingeborg M van GeijlswijkY Geert van der Meer
Jun 19, 2007·Clinical Chemistry·Jacoline W BrinkmanStephan J L Bakker
Sep 18, 2007·Clinical Chemistry and Laboratory Medicine : CCLM·Stefanie SwobodaTorsten Hoppe-Tichy
Feb 2, 2008·The Journal of Antimicrobial Chemotherapy·Miruna D David, Martin J Gill
Oct 14, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Tiphaine LegrandVincent Jullien
May 5, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Satoshi WatabeYukio Suzuki
Sep 28, 2010·Biomedical Chromatography : BMC·Christel GrondinEvelyne Jacqz-Aigrain
Mar 16, 2011·The Journal of Antimicrobial Chemotherapy·Cathrine McKenzie
Mar 25, 2011·Clinical Chemistry and Laboratory Medicine : CCLM·Samuel N CheuvrontMichael N Sawka
Sep 15, 2011·Critical Care : the Official Journal of the Critical Care Forum·Joao Gonçalves-Pereira, Pedro Póvoa
Oct 11, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Michael Cohen-WolkowiezAngela D M Kashuba
Jan 31, 2012·Clinical Biochemistry·Christiane OddozeHenri Portugal
Jan 29, 2013·Critical Care Medicine·R Phillip DellingerUNKNOWN Surviving Sepsis Campaign Guidelines Committee including the Pediatric Subgroup
Sep 10, 2013·International Journal of Antimicrobial Agents·Stefanie M Bode-BögerPeter Mailänder
Oct 26, 2013·Clinical Chemistry and Laboratory Medicine : CCLM·Johannes ZanderMichael Vogeser
Dec 12, 2013·Clinical Chemistry and Laboratory Medicine : CCLM·Johannes ZanderDaniel Teupser
Apr 29, 2014·The Lancet Infectious Diseases·Jason A RobertsUNKNOWN International Society of Anti-Infective Pharmacology and the Pharmacokinetics and Pharmacodynamics Study Group of the Europe
Jul 12, 2014·Critical Care : the Official Journal of the Critical Care Forum·Michael ZollerJohannes Zander

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Citations

Jan 28, 2017·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jens Martens-Lobenhoffer, Stefanie M Bode-Böger
Jul 29, 2017·Drug Testing and Analysis·Nahed El-NajjarAndré Gessner
Feb 16, 2021·Journal of Pharmaceutical and Biomedical Analysis·Sophie RaketeMichael Vogeser
Oct 23, 2020·Clinical Medicine Insights. Cardiology·Francesco NappiIrina Timofeeva

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