Effects of biomaterial-derived fibroblast conditioned medium on the α-amylase expression of parotid gland acinar cells

Acta Biomaterialia
Ya-Shuan ChouPei-Jen Lou

Abstract

Salivary gland cells are surrounded by a complex stromal environment, in which fibroblasts are the main cells in proximity to the gland cells. In this study, the interaction between parotid gland acinar cells (PGACs), fibroblasts, and biomaterials was investigated. We prepared different biomaterials, including chitosan, polyvinyl alcohol (PVA), poly (ethylene-co-vinyl alcohol) (EVAL), polyvinylidene fluoride (PVDF), and tissue culture polystyrene (TCPS) to culture fibroblasts and then collect their conditioned media to culture PGACs. We observed no difference in AQP3, AQP5, and E-cadherin expression among different fibroblast conditioned medium treatments. Interestingly, α-amylase expression was obviously enhanced in PGACs cultured in the presence of conditioned medium from fibroblasts cultured on PVDF. Higher neurotrophin-4 (NT-4) expression was observed in PVDF-derived fibroblast conditioned medium using a growth factor protein array assay. In addition, directly adding NT-4 into the culture medium significantly promoted α-amylase expression by PGACs. Finally, nestin and βIII-tubulin expression by fibroblasts cultured on PVDF was also enhanced. Together, these results suggest that PVDF could promote α-amylase expression by PGA...Continue Reading

References

Nov 1, 1995·The Journal of Cell Biology·S MiyamotoK M Yamada
Jan 1, 1996·Seikagaku. The Journal of Japanese Biochemical Society·Y FurukawaK Hayashi
Jan 1, 1996·Annual Review of Neuroscience·G R Lewin, Y A Barde
May 20, 1998·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·J C De VicenteJ A Vega
Mar 26, 1999·Journal of Cellular Physiology·C ZhengB C O'Connell
Sep 26, 2002·Proceedings of the National Academy of Sciences of the United States of America·Howard Y ChangPatrick O Brown
Jun 12, 2003·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·A VissinkR P Coppes
Apr 28, 2004·Current Opinion in Genetics & Development·Nastaran Zahir, Valerie M Weaver
Jun 21, 2005·Journal of Biomedical Materials Research. Part a·Min-Huey ChenTai-Horng Young
Aug 4, 2006·Biomaterials·Jeffrey C MohrSean P Palecek
Jan 20, 2007·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Xiangmei ChenQuan Hong
May 31, 2007·The British Journal of Dermatology·T WongH Navsaria
May 13, 2008·Tissue Engineering. Part B, Reviews·Doron J Aframian, Aaron Palmon
Jun 3, 2010·Physiology & Behavior·Emilie K BeltzerDouglas A Granger
Jun 4, 2010·Pathology International·Yuka KishabaToshiro Niki
Feb 21, 2013·Nature Communications·Sarah M KnoxMatthew P Hoffman

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Citations

Apr 3, 2016·Advanced Drug Delivery Reviews·Su Ryon ShinAli Khademhosseini
Mar 21, 2021·Communications Biology·Yuanhui SongDanielle S W Benoit

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