Effects of BNN27, a novel C17-spiroepoxy steroid derivative, on experimental retinal detachment-induced photoreceptor cell death

Scientific Reports
Pavlina TsokaMiltiadis K Tsilimbaris

Abstract

Retinal detachment (RD) leads to photoreceptor cell death secondary to the physical separation of the retina from the underlying retinal pigment epithelium. Intensifying photoreceptor survival in the detached retina could be remarkably favorable for many retinopathies in which RD can be seen. BNN27, a blood-brain barrier (BBB)-permeable, C17-spiroepoxy derivative of dehydroepiandrosterone (DHEA) has shown promising neuroprotective activity through interaction with nerve growth factor receptors, TrkA and p75NTR. Here, we administered BNN27 systemically in a murine model of RD. TUNEL+ photoreceptors were significantly decreased 24 hours post injury after a single administration of 200 mg/kg BNN27. Furthermore, BNN27 increased inflammatory cell infiltration, as well as, two markers of gliosis 24 hours post RD. However, single or multiple doses of BNN27 were not able to protect the overall survival of photoreceptors 7 days post injury. Additionally, BNN27 did not induce the activation/phosphorylation of TrkAY490 in the detached retina although the mRNA levels of the receptor were increased in the photoreceptors post injury. Together, these findings, do not demonstrate neuroprotective activity of BNN27 in experimentally-induced RD. ...Continue Reading

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Citations

Sep 6, 2019·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Alejandro Gonzalez-De la RosaArturo Santos
Sep 13, 2019·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Ruth Ibán-AriasKyriaki Thermos
Jun 9, 2020·Psychopharmacology·Nikolaos KokrasChristina Dalla
Jan 13, 2021·Brain Research Bulletin·Zi-Yuan ZhangGuang-Yu Li
Feb 15, 2021·Journal of Neuroscience Research·Ilias KalafatakisDomna Karagogeos

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Methods Mentioned

BETA
laser capture microdissection
electron microscopy
PCR
Assay

Software Mentioned

ImageJ
Zeiss ZEN
GraphPad Prism
Image J

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