PMID: 3763372Sep 1, 1986Paper

Effects of botulinum toxin induced muscle paralysis on endocytosis and lysosomal enzyme activities in mouse skeletal muscle

Pflügers Archiv : European journal of physiology
S TågerudS Thesleff

Abstract

The effects of botulinum toxin (type A) induced muscle paralysis on endocytosis and lysosomal enzyme activities in skeletal muscle were compared with the effects of surgical denervation. Muscle atrophy, measured as decrease in total muscle protein content, was as large or larger after botulinum toxin treatment as after denervation. Endocytic activity, measured as the in vitro uptake of horseradish peroxidase, and the specific activities of the lysosomal enzymes N-acetyl-beta-D-glucosaminidase and cathepsin D were all increased six days after denervation. Only the specific activity of cathepsin D was increased six days after botulinum toxin poisoning. The uptake of horseradish peroxidase and the specific activity of N-acetyl-beta-D-glucosaminidase were also increased eleven days after poisoning. Transverse sections of eleven days botulinum poisoned muscles from animals injected with horseradish peroxidase showed fibres with dense peroxidase staining similar to those seen in denervated muscle although they seemed to occur less frequently. The results show that increases in endocytic activity and lysosomal enzyme activities may occur in skeletal muscle without the presence of degenerating axons. The differences in effects of surgi...Continue Reading

References

Dec 1, 1977·The Biochemical Journal·W Schwartz, J W Bird
Sep 1, 1978·The Journal of Physiology·D A Mathers, S Thesleff
Jan 1, 1975·Journal of Neural Transmission·R Libelius
Nov 1, 1984·Journal of the Neurological Sciences·R Libelius, S Tågerud
Jun 1, 1960·The Journal of Physiology·S THESLEFF
Aug 1, 1949·The Journal of Physiology·A S V BURGENL J ZATMAN
Mar 15, 1949·The Journal of Physiology·N Ambache

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