Effects of botulinum toxin type D on secretion of tumor necrosis factor from human monocytes.

Molecular and Cellular Biology
K ImamuraD Kufe

Abstract

Botulinum toxins are potent neurotoxins which block the release of neurotransmitters. The effects of these toxins on hematopoietic cells, however, are unknown. Monocytes secrete a variety of polypeptide growth factors, including tumor necrosis factor (TNF). In the study reported here, the effects of botulinum toxin type D on the secretion of TNF from human monocytes were examined. The results demonstrate that botulinum toxin type D inhibits the release of TNF from monocytes activated by lipopolysaccharide (LPS) but not by 12-O-tetradecanoylphorbol-13-acetate. Botulinum toxin type D had no detectable effect on intracellular TNF levels in LPS-treated monocytes, indicating that the effects of this toxin involve the secretory process. This inhibitory effect of botulinum toxin type D on TNF secretion from LPS-treated monocytes was partially reversed by treatment with 12-O-tetradecanoylphorbol-13-acetate or introduction of guanosine 5'-[gamma-thio]triphosphate into these cells. The results demonstrate that TNF secretion is regulated by at least two distinct guanine nucleotide-binding proteins, one responsible for the activation of phospholipase C and another which acts as a substrate for botulinum toxin type D. ADP-ribosylation of mo...Continue Reading

References

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Citations

Mar 19, 2005·Pain Medicine : the Official Journal of the American Academy of Pain Medicine·Sota Omoigui, Sunday Irene
Dec 10, 2003·Oncogene·Deepa SampathWilliam Plunkett
Apr 25, 2002·Molecular and Cellular Biology·Kiyotsugu YoshidaDonald Kufe
Feb 26, 2020·Toxicon : Official Journal of the International Society on Toxinology·Bernard PoulainMichel R Popoff

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