Effects of bovine tumor necrosis factor alpha decoy receptors on cell death and inflammatory cytokine kinetics: potential for bovine inflammation therapy

BMC Veterinary Research
Sotaro FujisawaKazuhiko Ohashi

Abstract

Refractory diseases, including bacterial infections, are causing huge economic losses in dairy farming. Despite efforts to prevent and treat those diseases in cattle, including the use of antimicrobials, it is not well controlled in the field. Several inflammatory cytokines, including tumor necrosis factor alpha (TNF-α), play important roles in disease progression; thus, blocking these cytokines can attenuate the acute and sever inflammation and may be a novel strategy for treatment. However, biological drugs targeting inflammatory cytokines have not been used in cattle. Therefore, in this study, bovine sTNFR1 and sTNFR2 IgG1 Fc-fusion proteins (TNFR1-Ig and TNFR2-Ig) were produced, and their anti-inflammatory functions were analyzed in vitro, to develop decoy receptors for bovine TNF-α. Both TNFR1-Ig and TNFR2-Ig were shown to bind with TNF-α, and TNFR2-Ig showed higher affinity toward TNF-α than TNFR1-Ig. We next stimulated murine fibroblast-derived cells (L929 cells) with TNF-α to induce cell death and analyzed cell viability in the presence of TNFR-Ig proteins. Both TNFR1-Ig and TNFR2-Ig suppressed TNF-α-induced cell death, significantly improving cell viability. In addition, cell death induced by TNF-α was suppressed, even...Continue Reading

References

Mar 30, 1984·Journal of Immunological Methods·D A Flick, G E Gifford
Oct 1, 1993·The Journal of Infectious Diseases·T van der PollS J van Deventer
Jan 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·M GrellP Scheurich
Aug 8, 1998·Trends in Cell Biology·D J Van AntwerpD R Green
Oct 20, 1999·Cytokine·D T Humphreys, M R Wilson
Mar 13, 2001·Annual Review of Immunology·M Feldmann, R N Maini
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Vincent CottinDavid W H Riches
Nov 2, 2002·Kidney International. Supplement·Manuel Praga, Enrique Morales
Mar 26, 2003·Cell Death and Differentiation·H WajantP Scheurich
Sep 2, 2003·Nature Reviews. Immunology·Bharat B Aggarwal
Oct 30, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Georg H WaetzigStefan Schreiber
Jan 30, 2007·Inflammatory Bowel Diseases·Tanja SpoettlGerhard Rogler
Sep 5, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marie BenoitJean-Louis Mege
Jun 30, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Weidong JiWang Min
Mar 25, 2015·Veterinary Immunology and Immunopathology·Satoshi GondairaHajime Nagahata
Jan 2, 2016·The Journal of Biological Chemistry·Isabell LangHarald Wajant
May 19, 2016·Immunity·Lindsay K Ward-KavanaghCarl F Ware
Jul 18, 2016·Trends in Immunology·Adrian T Ting, Mathieu J M Bertrand
Sep 14, 2016·Cytokine·Eugene Varfolomeev, Domagoj Vucic
Oct 1, 2016·The Veterinary Journal·Robin A J NicholasInna Lysnyansky
Jan 24, 2018·Frontiers in Veterinary Science·Chetan SharmaHarsh Panwar

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Citations

Aug 29, 2021·Veterinary Research Communications·Pamela Anahí LendezMaría Carolina Ceriani

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Datasets Mentioned

BETA
NM001040490

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA
Assay
PCR
ubiquitination
density gradient centrifugation

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