Effects of brain necroptosis and cytokines expression array on brain injury in rats with cardiac arrest

Zhonghua wei zhong bing ji jiu yi xue
Jiajun XuJianli Yao

Abstract

To investigate the underlying molecular mechanisms of brain injury in rats after cardiac arrest and cardiopulmonary resuscitation (CPR) by observing necroptosis of brain cells and changes of 90 cytokines in brain tissue. Sprague-Dawley (SD) rats were divided into Sham group (n = 10) and cardiac arrest group (n = 10) according to random number table method. The model of asphyxia cardiac arrest for 6 minutes followed by CPR model was established. Tracheal intubation in Sham rats were routinely performed without inducing cardiac arrest. Neurological deficit score (NDS) was evaluated, blood samples were collected and rats were sacrificed, then serum S100B level was measured by enzyme linked immunosorbent assay (ELISA) on the third day after CPR. Necroptotic cells in brain were detected by immunofluorescence staining. The levels of 90 cytokines expression in brain were measured by antibody array. The relative ratio of the two groups of protein expression ≥ 1.5 or ≤ 0.5 and P < 0.05 represented the differential expression protein. There were 8 rats successfully resuscitated and 2 died in cardiac arrest group. There were 8 rats selected in Sham group to match the sample size. Compared with Sham group, the NDS score of cardiac arrest g...Continue Reading

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