Dec 1, 1987

Effects of bumetanide on chloride transport in human eccrine sweat ducts: implications for cystic fibrosis

Israel Journal of Medical Sciences
M M Reddy, P M Quinton


The effect of the loop diuretic bumetanide on Cl- transport in the human reabsorptive sweat duct (RSD) was studied to determine the properties of Cl- transport in this tissue with an emphasis on its role in cystic fibrosis. Bumetanide (10(-4) M) in the bath decreased the NaCl reabsorption rate (+/- SE) from 235 +/- 72 to 77 +/- 24 pmol/min per mm (n = 10). Bumetanide in the lumen decreased the rate of NaCl reabsorption to a lesser extent from 132 +/- 17 to 84 +/- 16 pmol/min per mm (n = 9). At least part of this inhibition appears to be due to the inhibition of a Cl- conductance in the RSD, as evidenced by a significant increase in the specific resistance (Rt) of the tissue from 8.3 +/- 0.6 to 52.7 +/- 19.3 omega/cm2(n = 3) due to bumetanide in the bath. Luminal bumetanide also increased Rt but to a lesser degree [from 9.8 +/- 1.2 to 16.4 +/- 2.0 omega/cm2 (n = 3)]. Bumetanide also affected the transepithelial potential (Vt), hyperpolarizing it from -10.9 +/- 0.8 to -28.6 +/- 2.0 mV (n = 13). In ouabain-inhibited ducts, a threefold dilution of luminal NaCl concentration resulted in a -13.5 +/- 1.7 mV dilution diffusion potential which was abolished by bumetanide in the bath, indicating that bumetanide removed the anion selectiv...Continue Reading

  • References
  • Citations


  • We're still populating references for this paper, please check back later.
  • References
  • Citations


  • This paper may not have been cited yet.

Mentioned in this Paper

Entire Duct
Biochemical Pathway
Entire Lumen of Body System
Eccrine Glands
Specimen Type - Sweat
Resting Potentials
Process of Absorption

About this Paper

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Bone Marrow Neoplasms

Bone Marrow Neoplasms are cancers that occur in the bone marrow. Discover the latest research on Bone Marrow Neoplasms here.

IGA Glomerulonephritis

IgA glomerulonephritis is a chronic form of glomerulonephritis characterized by deposits of predominantly Iimmunoglobin A in the mesangial area. Discover the latest research on IgA glomerulonephritis here.

Cryogenic Electron Microscopy

Cryogenic electron microscopy (Cryo-EM) allows the determination of biological macromolecules and their assemblies at a near-atomic resolution. Here is the latest research.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

LRRK2 & Immunity During Infection

Mutations in the LRRK2 gene are a risk-factor for developing Parkinson’s disease. However, LRRK2 has been shown to function as a central regulator of vesicular trafficking, infection, immunity, and inflammation. Here is the latest research on the role of this kinase on immunity during infection.

Antiphospholipid Syndrome

Antiphospholipid syndrome or antiphospholipid antibody syndrome (APS or APLS), is an autoimmune, hypercoagulable state caused by the presence of antibodies directed against phospholipids.

Meningococcal Myelitis

Meningococcal myelitis is characterized by inflammation and myelin damage to the meninges and spinal cord. Discover the latest research on meningococcal myelitis here.

Alzheimer's Disease: MS4A

Variants within membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease by recent genome-wide association studies. Here is the latest research.