Effects of cadmium exposure on expression of glutathione synthetase system genes in Acidithiobacillus ferrooxidans

Extremophiles : Life Under Extreme Conditions
Chunli ZhengRen Fang Shen

Abstract

The glutathione synthetase system (GSS) is an important pathway of glutathione synthesis and plays a key role in heavy metal resistance. In this work, the response of Acidithiobacillus ferrooxidans to extracellular Cd2+ was investigated, and the interplay between Cd2+ resistance and the expression of GSS related-genes was analyzed by reverse-transcription quantitative PCR (RT-PCR). During growth in the presence of 5, 15 and 30 mM Cd2+, the transcript levels of eight GSS pathway genes were affected between 0.81- and 7.12-fold. Increased transcription was also reflected in increased enzyme activities: with those of glutathione reductase (GR) increased by 1.10-, 2.26- and 1.54-fold in the presence of 5, 15 and 30 mM Cd2+, respectively. In contrast, the activities of catalase (CAT) and superoxide dismutase (SOD) were decreased in the presence of Cd2+. At the metabolite level, intracellular methane dicarboxylic aldehyde (MDA) content was increased 1.97-, 3.31- and 1.92-fold in the presence of 5, 15 and 30 mM Cd2+, respectively. These results suggest that Cd2+ directly inhibits the activities of CAT and SOD, breaks the redox balance of the cells, which leads to the activation of the other antioxidant pathway of GSS. Resistance of A. ...Continue Reading

References

Jan 1, 1990·Methods in Enzymology·H H Draper, M Hadley
Nov 1, 1971·Analytical Biochemistry·C Beauchamp, I Fridovich
Jul 1, 1993·British Medical Bulletin·K H Cheeseman, T F Slater
Aug 2, 2005·Nutrition, Metabolism, and Cardiovascular Diseases : NMCD·Daniele Del RioNicoletta Pellegrini
Jul 4, 2007·Environmental Toxicology·Prashant MohanpuriaSudesh Kumar Yadav
Mar 3, 2012·Journal of Clinical Medicine Research·Kazuhiko Kotani, Nobuyuki Taniguchi
Apr 11, 2012·International Journal of Molecular Sciences·Marijke JozefczakAnn Cuypers
Dec 4, 2012·Biochimica Et Biophysica Acta·Regina Brigelius-Flohé, Matilde Maiorino
May 21, 2013·Plant Science : an International Journal of Experimental Plant Biology·Gisele PassaiaMarcia Margis-Pinheiro
Oct 15, 2013·Free Radical Biology & Medicine·Venkat R PannalaRanjan K Dash
Oct 31, 2013·Scientific Reports·Tadayuki IwaseYoshimitsu Mizunoe
Mar 1, 2016·Free Radical Biology & Medicine·Narciso CoutoJill Barber
Nov 3, 2016·Biological Trace Element Research·Hacer DoganFikret Karatas

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Citations

Apr 6, 2019·Scientific Reports·Xiaolin LiBo Zhang

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Methods Mentioned

BETA
electrophoresis
PCR
MDA
environmental stresses

Software Mentioned

Primer Premier

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