Effects of cadmium on electrolyte ions in cultured rat hepatocytes studied by X-ray microanalysis of cryosections

Toxicology and Applied Pharmacology
K Zierold

Abstract

The distribution of elements in isolated and cultured rat hepatocytes was measured by energy dispersive electron probe X-ray microanalysis of freeze-dried ultrathin cryosections. The intracellular compartmentation of electrolyte ions, in particular the content of sodium, chloride, and potassium, was found to depend on culture conditions and on the amount of cadmium chloride added to the culture medium. In cells exposed to 1-10 microM cadmium without carbon dioxide supply, the potassium/sodium ratio decreased from control values of about 10 to values below 1 within 30 min. Changes of potassium and sodium content were followed by an increase in the intracellular chloride content. In cells exposed to 1-10 microM cadmium with carbon dioxide supply, changes of the electrolyte composition were delayed to 1-2 days. An increase of intracellular chloride preceded the inversion of the intracellular potassium/sodium ratio. High cadmium doses induced a cytoplasmic calcium increase and finally disintegration and decay of cell structure. Almost normal potassium and sodium contents were found in cells exposed to 10 microM cadmium in the presence of 100 microM zinc with carbon dioxide for 1 day. Changes in the intracellular electrolyte composi...Continue Reading

References

Mar 1, 1977·Toxicology and Applied Pharmacology·E J FaederB A Fowler
Jun 1, 1975·Hoppe-Seyler's Zeitschrift für physiologische Chemie·H BaurE Pfaff
Jan 1, 1990·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·J LiuC D Klaassen
Jan 1, 1989·Toxicology and Applied Pharmacology·B F TrumpK A Elliget
Jun 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·E PetzingerR K Kinne
May 1, 1988·Journal of Electron Microscopy Technique·K Zierold
Jun 1, 1984·Toxicology·L Müller, F K Ohnesorge
Aug 18, 1995·The Science of the Total Environment·M TorraJ Corbella
May 1, 1992·Toxicology in Vitro : an International Journal Published in Association with BIBRA·G CarreraP Martel
Nov 1, 1992·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J P GrotenP J van Bladeren

❮ Previous
Next ❯

Citations

Apr 9, 2005·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Karl ZieroldGérard Balossier
Dec 15, 2011·Tissue Engineering. Part C, Methods·Ingrid GarzónMiguel Alaminos
Oct 22, 2008·American Journal of Respiratory Cell and Molecular Biology·Franck DelavoieGérard Balossier
May 10, 2008·Annals of Vascular Surgery·A Rodriguez-MorataA Campos
Jan 13, 2006·Toxicology Letters·George Fotakis, John A Timbrell
Jul 12, 2008·Methods in Cell Biology·E Fernandez-Segura, Alice Warley
May 23, 2003·Journal of Biomedical Materials Research. Part a·Patrice LaquerriereGérard Balossier
Feb 18, 2005·Journal of Cellular Physiology·Francisco ArrebolaEduardo Fernández-Segura
Sep 18, 2007·Biological Trace Element Research·T NagamineK Arakawa
Oct 11, 2005·Apoptosis : an International Journal on Programmed Cell Death·F ArrebolaE Fernández-Segura
Jul 12, 2019·Environmental and Molecular Mutagenesis·Daniel J SmartPatrick Vanscheeuwijck
Oct 18, 2013·Experimental Biology and Medicine·Ana Celeste OliveiraMiguel Alaminos
Oct 18, 2000·Toxicology in Vitro : an International Journal Published in Association with BIBRA·K Zierold
Mar 1, 2002·Toxicology in Vitro : an International Journal Published in Association with BIBRA·S Bolkent, K Zierold

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.