PMID: 11926612Apr 3, 2002Paper

Effects of cadmium on gap junctional intercellular communication in WB-F344 rat liver epithelial cells

Human & Experimental Toxicology
S H JeonJ H Cho

Abstract

Cadmium has been associated with a number of tumors but its role in tumor promotion has not been elucidated clearly or the results obtained from various studies have been conflicting. This study was designed to investigate the effects of cadmium on the gap junctional intercellular communication (GJIC), number of gap junctions per cell, and cell proliferation in WB-F344 rat liver epithelial cells from the viewpoint of tumor promotion. GJIC was monitored by counting the cells stained with Lucifer yellow CH dye, using the scrape-loading and dye-transfer method. The numbers of gap junctions per cell were visually quantitated after an indirect immunostaining for gap junction protein using an antibody to connexin 43. Cell proliferation was assayed by direct counting of the living cells using the trypan blue dye exclusion method. In the time course study, cells treated with 200 microM CdCl2 showed rapid and nearly complete inhibition of GJIC (approximately 14% of the control) and a decrease in the number of gap junctions per cell (approximately 21% of the control) at 30 min, and the decrease continued up to 4 h without any changes in the cell viability. Treatment with CdCl2 (7.4-200 microM) for 4 h resulted in the decrease of GJIC and...Continue Reading

References

Feb 4, 1979·Biochimica Et Biophysica Acta·W R Loewenstein
Dec 1, 1991·The American Journal of Physiology·J D Clark, L E Limbird
Mar 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·D ZhuC C Naus
Dec 1, 1990·The American Review of Respiratory Disease·W R Loewenstein
May 3, 1988·Biochemistry·Y Yarden, A Ullrich
Dec 1, 1987·Environmental Health Perspectives·J C Barrett, R W Wiseman
May 29, 1987·Biochemical and Biophysical Research Communications·U N DasD F Horrobin
Oct 1, 1981·Physiological Reviews·W R Loewenstein
Sep 1, 1994·Environmental Health Perspectives·D BeyersmannA N Malviya
Dec 1, 1995·Toxicology Letters·G A BoormanR A Herbert
Aug 31, 2000·Toxicological Sciences : an Official Journal of the Society of Toxicology·S H JeongC D Klaassen
Jan 1, 1990·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J E TroskoB V Madhukar

❮ Previous
Next ❯

Citations

Dec 4, 2003·Mutation Research·Michael P Waalkes
Sep 28, 2010·Chemical Research in Toxicology·Mathieu VinkenVera Rogiers
Jan 18, 2011·Antioxidants & Redox Signaling·Xin FangJian Yao
Nov 1, 2011·Pharmacological Reviews·C Yan Cheng, Dolores D Mruk
Jun 29, 2010·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Emmy Van KerkhoveQuirine Swennen
Oct 15, 2013·Biology of the Cell·Sylviane BoucherieLaurent Combettes
Feb 9, 2017·Journal of Applied Toxicology : JAT·Qingping LiuQing Wu
Jun 27, 2017·Environmental Toxicology and Pharmacology·Zehe GeQing Wu
Mar 3, 2010·Journal of Veterinary Science·Hwan-Goo KangJoon-Hyoung Cho
Jul 29, 2009·Critical Reviews in Biochemistry and Molecular Biology·Mathieu VinkenVera Rogiers
Jun 23, 2020·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Kaat LeroyMathieu Vinken
Aug 28, 2021·International Journal of Molecular Sciences·Iva SovadinováPavel Babica

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.