Effects of cadmium on the co-ordination of nitrogen and carbon metabolism in bean seedlings

Journal of Plant Physiology
Houda GouiaMohamed Habib Ghorbal

Abstract

The effect of cadmium (Cd) was investigated on the in vitro activities of leaf and root enzymes involved in carbon (C) and nitrogen (N) metabolism of bean (Phaseolus vulgaris L. cv. Morgane). Cd induced a high increase in maximal extractable activity of glutamate dehydrogenase (NADH-GDH, EC 1.4.1.2). Cd promoted ammonium accumulation in leaves and roots, and a tight correlation was observed between ammonium amount and GDH activity. Changes in GDH activity appear to be mediated by the increase in ammonium levels by Cd treatment. Cd stress also enhanced the activities of phosphoenolypyruvate carboxylase (PEPC, EC 4.1.1.31) and NADP(+)-isocitrate dehydrogenase (NADP(+)-ICDH, EC 1.1.1.42) in leaves while they were inhibited in roots. Immuno-titration, the PEPC sensitivity to malate and PEPC response to pH indicated that the increase in PEPC activity by Cd was due to de novo synthesis of the enzyme polypeptide and also modification of the phosphorylation state of the enzyme. Cd may have modified, via a modulation of PEPC activity, the C flow towards the amino acid biosynthesis. In leaves, Cd treatments markedly modified specific amino acid contents. Glutamate and proline significantly accumulated compared to those of the control pla...Continue Reading

References

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Citations

Feb 5, 2013·Journal of Plant Physiology·Ewa GajewskaMaria Skłodowska
Jan 15, 2013·Plant Physiology and Biochemistry : PPB·Sarvajeet Singh GillNarendra Tuteja
Sep 26, 2015·Environmental Science and Pollution Research International·Peng WangCunyi Yang
Feb 3, 2009·Journal of Plant Physiology·Ewa Gajewska, Maria Skłodowska
Mar 10, 2010·Physiologia Plantarum·David Martínez DomínguezRafael Torronteras Santiago
Aug 30, 2005·Comptes rendus biologies·Nabil Ben YoussefDouja Ben Miled Daoud
May 4, 2018·International Journal of Phytoremediation·Rahat ShabirFarah Ashraf
Jan 17, 2013·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Kusum VermaG S Shekhawat
Jul 29, 2011·Biological Trace Element Research·Savita GangwarJagat Narayan Maurya
Sep 7, 2016·International Journal of Phytoremediation·A M Shackira, Jos T Puthur
Aug 27, 2018·Environmental Science and Pollution Research International·Peng WangCunyi Yang
Jan 9, 2019·Frontiers in Plant Science·Sladjana SamuilovHeinz Rennenberg
Nov 23, 2020·Environmental Science and Pollution Research International·Senlin YangLianghua Chen

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