Effects of caffeine supplementation in post-thaw human semen over different incubation periods

Andrologia
J R Pariz, J Hallak

Abstract

This study aimed to evaluate the effects of caffeine supplementation in post-cryopreservation human semen over different incubation periods. After collection by masturbation, 17 semen samples were analysed according to World Health Organization criteria, processed and cryopreserved with TEST-yolk buffer (1 : 1) in liquid nitrogen. After a thawing protocol, samples were incubated with 2 mm of caffeine for 0, 5, 15, 30 or 60 min, followed by analysis of motility and mitochondrial activity using 3,3'-diaminobenzidine (DAB). Mean variance analysis was performed, and P < 0.05 was the adopted significance threshold. Samples incubated for 15 min showed increased progressive motility compared to other periods of incubation, as well as a reduced percentage of immotile spermatozoa (P < 0.05). In samples incubated for 5 min, increased mitochondrial activity above 50% was observed (DABI and DABII). Although cryosurvival rates were low after the cryopreservation process, incubation with caffeine was associated with an increase in sperm motility, particularly 15-min incubation, suggesting that incubation with caffeine can be an important tool in patients with worsening seminal quality undergoing infertility treatment.

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Citations

Sep 19, 2018·Zygote : the Biology of Gametes and Early Embryos·João Diego de Agostini LosanoMarcilio Nichi
Nov 29, 2017·Zygote : the Biology of Gametes and Early Embryos·Cleiton WernerMarco Aurélio Echart Montano
Jul 16, 2020·Human Fertility : Journal of the British Fertility Society·Abigail SharpeDavid Miller
Jul 3, 2021·Journal of Clinical Medicine·Kaan Aydos, Oya Sena Aydos

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