PMID: 6105882Aug 4, 1980Paper

Effects of calcium and soluble cytoplasmic activator protein (calmodulin) on various states of (Ca2+ + Mg2+)-ATPase activity in isolated membranes of human red cells

Biochimica Et Biophysica Acta
M G Luthra, H D Kim

Abstract

(Ca2+ + Mg2+)-ATPase activity of red cells and their isolated membranes was investigated in the presence of various Ca2+ concentrations and cytoplasmic activator protein. Red cell ATPase activity was high at low Ca2+ concentrations, and low at moderate and high concentrations of Ca2+. In the case of isolated membranes, both low and moderate ca2+ concentrations produced higher (Ca2+ + Mg2+)-ATPase activity than high Ca2+ concentration. Membrane-free hemolysate containing soluble activator of (Ca2+ + Mg2+)-ATPase produced a significant increase in (Ca2+ + Mg2+)-ATPase activity only at low ca2+ concentration. Regardless of Ca2+ and activator concentrations, the enzyme activity in the membrane was lower than lysed red cells. The low level of (Ca2+ + Mg2+)-ATPase activity seen at high Ca2+ concentration can be augmented by lowering the Ca2+ concentration of EGTA in the assay medium. However, once the membrane was exposed to a high Ca2+ concentration, the activator could no longer exert it maximum stimulation at the low Ca2+ concentration brought about by addition of EGTA. This loss of activation was not attributable to the Ca2+-induced denaturation of activator protein but rather related to the alteration of (Ca2+ + Mg2+)-ATPase sta...Continue Reading

References

Jan 8, 1976·Biochimica Et Biophysica Acta·M G LuthraD J Hanahan
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Citations

Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M VolpiM B Feinstein
Nov 20, 1981·Biochimica Et Biophysica Acta·S Thompson, A H Maddy
Aug 27, 2015·PloS One·Kristen M PluchinoMichael M Gottesman
Mar 30, 1984·Biochemical and Biophysical Research Communications·H D KimR E Isaacks
Aug 1, 1985·European Journal of Clinical Investigation·U Walter, S Müller
Jan 1, 1982·Annals of the New York Academy of Sciences·F F Vincenzi
Jan 1, 1982·Annals of the New York Academy of Sciences·B SarkadiG Gárdos
Sep 21, 1981·Biochimica Et Biophysica Acta·S Muallem, S J Karlish

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