PMID: 6247493Jan 1, 1980

Effects of calcium, lanthanum, and temperature on the fluidity of spin-labeled human platelets

The Journal of Membrane Biology
R D SauerheberL M Gordon

Abstract

Previous platelet studies have shown that calcium plays important roles in stimulus-secretion coupling, aggregation, and other membrane-associated functions. In addition, lanthanum induces platelet aggregation and the platelet release reaction and also influences platelet responsiveness to various stimuli. The spin-label results presented here suggest that one mechanism through which calcium and lanthanum mediate their effects on platelet functions may be by decreasing the lipid fluidity of the surface membrane. The structure of platelet membrane lipids was examined with the spin-label method. Washed human platelets were labeled with the 5-, 12- and 16-nitroxide stearic acid spin probes. Order parameters which measure the fluidity of the lipid environment of the incorporated probe may be calculated from the electron spin resonance (ESR) spectra of 5-nitroxide stearate [I(12,3)]-labeled cells. Evidence is presented which indicates that these spectra principally reflect properties of the platelet surface membrane lipids. The membrane fluidity increased with temperature for the range 17 to 37 degrees C. Either calcium or lanthanum additions to intact cells increased the rigidity of the platelet membranes at 37 degrees C, although ...Continue Reading

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Related Concepts

Calcium
Spin Labels
Platelet Aggregation
Egtazic Acid Sodium Salt
Lanthanum
Membrane Fluidity
Ferricyanides
Blood Platelets

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