Aug 1, 1984

Effects of carbon dioxide and pH variations in vitro on blood respiratory functions, red blood cell volume, transmembrane pH gradients, and sickling in sickle cell anemia

The Journal of Laboratory and Clinical Medicine
Y Ueda, R M Bookchin

Abstract

Findings that polymerization of hemoglobin S is an oxygen-linked function, and that CO2 has an O2 affinity-independent effect on deoxyhemoglobin S polymerization suggests that varying PCO2 might have different effects on respiratory functions and other red blood cell properties of blood in sickle cell anemia (SS) compared with normal blood (AA). We examined the O2 affinity, Bohr effect, transmembrane pH gradient, mean cell hemoglobin concentration, and red blood cell sickling at half O2 saturation in whole SS and AA blood during CO2 titration and acid-base titration at three PCO2 levels, 10, 40, and 80 mm Hg. The CO2-induced Bohr effect of SS blood was considerably larger than normal (maximum, 0.91, referred to cell pH) and similar to that found with acid-base titration at PCO2 of 40. In contrast to AA blood, SS blood showed an increased O2 affinity when PCO2 was raised from 40 to 80, and at half O2 saturation showed biphasic or sigmoid Bohr curves, a fall in transmembrane pH gradient with rising PCO2, and an absence of the normal cell volume increase at low pH and PCO2. Sickling of SS cells at half O2 saturation was partly inhibited by increasing PCO2, particularly in the higher pH ranges. These complex differences in the beha...Continue Reading

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Mentioned in this Paper

Deoxyhemoglobin S
Dioxygen
Normal Cell
Sigmoid Colon
Protoplasm
Resting Potentials
DNAJB1
Hemoglobin SS
Drepanocyte (Cell)
Red Cell Mass Result

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