Abstract
The aim of this study was to test the effects of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) crème, or MI Paste™ (MIP), on nicotine-induced Streptococcus mutans biofilm. The experiment utilized S. mutans biofilm assays with varying concentrations of nicotine and MIP aqueous concentrate levels. First hand exposure to nicotine has been demonstrated to significantly increase S. mutans biofilm formation, while the active component, CPP-ACP, in MIP has been shown to reduce S. mutans biofilm formation. A 24-h culture of S. mutans UA159 in microtiter plates were treated with varying nicotine concentrations (0-32 mg/ml) in Tryptic Soy Broth supplemented with 1% sucrose (TSBS) with or without MIP aqueous concentrate. A spectrophotometer was used to determine total growth absorbance and planktonic growth. The microtiter plate wells were washed, fixed, and stained with crystal violet dye and the absorbance measured to determine biofilm formation. The presence of MIP aqueous concentrate inhibits nicotine-induced S. mutans biofilm formation at different concentrations of nicotine (0-32 mg/ml). The results demonstrated nicotine-induced S. mutans biofilm formation is decreased in the presence of MIP. This provides further evid...Continue Reading
References
Jan 1, 1992·Journal of General Microbiology·M C TrombeJ M Manias
Oct 1, 1996·Journal of Dental Research·P SchüpbachB Guggenheim
Sep 19, 1997·Journal of Dental Research·E C Reynolds
May 30, 1998·Journal of Clinical Periodontology·P AxelssonJ Lindhe
Oct 29, 1998·Special Care in Dentistry : Official Publication of the American Association of Hospital Dentists, the Academy of Dentistry for the Handicapped, and the American Society for Geriatric Dentistry·E C Reynolds
Apr 29, 2000·Archives of Oral Biology·R K Rose
Oct 3, 2000·Caries Research·R K Rose
Apr 2, 2002·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·K David Hay, W Murray Thomson
Sep 3, 2004·Community Dentistry and Oral Epidemiology·Poul Erik Petersen, Michael A Lennon
Jan 26, 2006·The Journal of Dairy Research·Glenn WalkerEric Reynolds
Sep 26, 2007·International Journal of Cancer. Journal International Du Cancer·Sara GandiniPeter Boyle
Mar 26, 2008·Journal of Dental Research·E C ReynoldsC Reynolds
May 1, 2008·Caries Research·M V MorganE C Reynolds
Aug 19, 2008·European Journal of Oral Sciences·Sheng-Fu HuangXing Long
Aug 25, 2009·Acta Odontologica Scandinavica·Veerasamy Yengopal, Steffen Mickenautsch
Jul 23, 2011·Virulence·Ruijie HuangRichard L Gregory
Aug 31, 2011·Journal of Dental Research·J LeeR Vassallo
Apr 1, 1989·The Behavior Analyst·J E Henningfield, S T Higgins
Nov 13, 2015·MMWR. Morbidity and Mortality Weekly Report·Ahmed JamalBrian A King