Effects of caulophine on caffeine-induced cellular injury and calcium homeostasis in rat cardiomyocytes

Basic & Clinical Pharmacology & Toxicology
Kaiwei SiXiaoqi Li

Abstract

Caulophine is a novel fluorenone alkaloid isolated from the radix of Caulophyllum robustum Maxim. Caulophine showed high affinity for the rat myocardial cell membrane as assessed by cell membrane chromatography, suggesting that the compound may exert bioactivity in the heart. It is known that calcium plays an important role in the pathogenesis of ischaemic heart disease, and caffeine can cause calcium overload in cardiomyocytes by inducing calcium release from the sarcoplasmic reticulum. Therefore, the present study evaluated the effects of caulophine on caffeine-induced injury and calcium homeostasis in cardiomyocytes. Cardiomyocytes were pre-treated with caulophine before exposure to caffeine or potassium chloride (KCl). Cell viability was assayed using the MTT method, and lactate dehydrogenase (LDH) and malondialdehyde (MDA) were measured spectrophotometrically. Caulophine-pre-treated cardiomyocytes were incubated with Fluo-3/AM, and then caffeine or KCl was used to induce Ca(2+) overload. The total intracellular Ca(2+) concentration was measured by flow cytometry. Fluorescence densities of single cardiomyocytes were detected using a confocal microscope. Caulophine increased the viability of caffeine-injured cardiomyocytes a...Continue Reading

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Citations

Jun 29, 2014·Journal of Pharmaceutical and Biomedical Analysis·Xiaofang HouLangchong He
May 31, 2014·Evidence-based Complementary and Alternative Medicine : ECAM·Yong-Gang XiaHai-Xue Kuang

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