Effects of CB2 and TRPV1 receptors' stimulation in pediatric acute T-lymphoblastic leukemia

Oncotarget
Francesca PunzoFrancesca Rossi

Abstract

T-Acute Lymphoblastic Leukemia (T-ALL) is less frequent than B-ALL, but it has poorer outcome. For this reason new therapeutic approaches are needed to treat this malignancy. The Endocannabinoid/Endovanilloid (EC/EV) system has been proposed as possible target to treat several malignancies, including lymphoblastic diseases. The EC/EV system is composed of two G-Protein Coupled Receptors (CB1 and CB2), the Transient Potential Vanilloid 1 (TRPV1) channel, their endogenous and exogenous ligands and enzymes. CB1 is expressed mainly in central nervous system while CB2 predominantly on immune and peripheral cells, therefore we chose to selectively stimulate CB2 and TRPV1. We treated T-ALL lymphoblasts derived from 4 patients and Jurkat cells with a selective agonist at CB2 receptor: JWH-133 [100 nM] and an agonist at TRPV1 calcium channel: RTX [5 uM] at 6, 12 and 24 hours. We analyzed the effect on apoptosis and Cell Cycle Progression by a cytofluorimetric assays and evaluated the expression level of several target genes (Caspase 3, Bax, Bcl-2, AKT, ERK, PTEN, Notch-1, CDK2, p53) involved in cell survival and apoptosis, by Real-Time PCR and Western Blotting. We observed a pro-apoptotic, anti-proliferative effect of these compounds in...Continue Reading

References

Feb 29, 2000·British Journal of Pharmacology·D SmartJ B Davis
Jun 12, 2003·Annals of the New York Academy of Sciences·Meritxell Alberich JordaRuud Delwel
Nov 28, 2006·Biochimica Et Biophysica Acta·James A McCubreyRichard A Franklin
Apr 10, 2009·Cell Calcium·Luciano De Petrocellis, Vincenzo Di Marzo
May 22, 2009·Immunobiology·Sadiye Amcaoglu RiederPrakash Nagarkatti
Jun 11, 2009·Acta Oncologica·Claudia BurzAlexandru Irimie
Jan 18, 2011·Bone·Francesca RossiUNKNOWN Endocannabinoid Research Group (ERG), Italy
Apr 16, 2011·Leukemia·L S SteelmanJ A McCubrey
Jul 13, 2011·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Jana HofRenate Kirschner-Schwabe
Oct 31, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Vincenzo Di Marzo, Luciano De Petrocellis
Feb 26, 2013·BMC Complementary and Alternative Medicine·Chia-Han LinMu-Kuan Chen
Apr 30, 2013·Current Cancer Drug Targets·Bing-Hua Jiang
May 2, 2013·Genes & Cancer·Mathew C CasimiroRichard G Pestell
Feb 15, 2014·Cellular and Molecular Life Sciences : CMLS·Mirko TantimonacoMauro Maccarrone
Jan 1, 2012·Cells·Karl Holmberg OlaussonMikael S Lindström
Aug 15, 2014·Oncotarget·Bandana ChakravartiRamesh K Ganju
Dec 2, 2014·Pediatric Clinics of North America·Deepa BhojwaniChing-Hon Pui
Jun 29, 2015·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Francesca RossiSabatino Maione
Sep 17, 2015·Nature Reviews. Neuroscience·Loren H Parsons, Yasmin L Hurd
Mar 30, 2016·Current Oncology·G VelascoM Guzmán
Apr 12, 2016·Indian Journal of Hematology & Blood Transfusion : an Official Journal of Indian Society of Hematology and Blood Transfusion·Cristian Fabián Layton Tovar, Hugo Mendieta Zerón
Aug 10, 2016·Current Hematologic Malignancy Reports·Lynda M Vrooman, Lewis B Silverman
Oct 26, 2016·Genes, Chromosomes & Cancer·Kristina Karrman, Bertil Johansson
Dec 7, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Giulia BelliniFrancesca Rossi
Jul 1, 2017·Blood Cancer Journal·T Terwilliger, M Abdul-Hay

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Citations

Jun 18, 2020·International Journal of Molecular Sciences·Kevin ZhaiDietrich Büsselberg
Jun 3, 2021·Biomolecules·Federica MaggiConsuelo Amantini

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Methods Mentioned

BETA
PCR
Assay

Software Mentioned

Image Studio Digit
StatGraphics Centurion XV
MUSE Cell Analyzer
Muse cell Analyser
Muse
Image studio Digits

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