PMID: 9525605Apr 3, 1998Paper

Effects of CCR5 and CD4 cell surface concentrations on infections by macrophagetropic isolates of human immunodeficiency virus type 1

Journal of Virology
E J PlattD Kabat

Abstract

It has been proposed that changes in cell surface concentrations of coreceptors may control infections by human immunodeficiency virus type 1 (HIV-1), but the mechanisms of coreceptor function and the concentration dependencies of their activities are unknown. To study these issues and to generate stable clones of adherent cells able to efficiently titer diverse isolates of HIV-1, we generated two panels of HeLa-CD4/CCR5 cells in which individual clones express either large or small quantities of CD4 and distinct amounts of CCR5. The panels were made by transducing parental HeLa-CD4 cells with the retroviral vector SFF-CCR5. Derivative clones expressed a wide range of CCR5 quantities which were between 7.0 x 10(2) and 1.3 x 10(5) molecules/cell as measured by binding antibodies specific for CCR5 and the chemokine [125I]MIP1beta. CCR5 was mobile in the membranes, as indicated by antibody-induced patching. In cells with a large amount of CD4, an unexpectedly low trace of CCR5 (between 7 x 10(2) and 2.0 x 10(3) molecules/cell) was sufficient for maximal susceptibility to all tested HIV-1, including primary patient macrophagetropic and T-cell-tropic isolates. Indeed, the titers as indicated by immunoperoxidase staining of infected ...Continue Reading

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Citations

Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·B LeeR W Doms
Feb 28, 2002·British Journal of Pharmacology·Anja MuellerPhilip G Strange
Oct 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Carsten MünkNathaniel R Landau
Nov 22, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jacqueline D ReevesRobert W Doms
Feb 8, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Seth H PincusWilliam C Olson
Nov 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yea-Lih LinPierre Corbeau
Jan 8, 2003·Reviews in Medical Virology·Katherine KedzierskaAnthony L Cunningham
Jan 7, 2004·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Rohan JohnDavid H Schwartz
Jun 15, 2007·Journal of Virology·Amapola ManriqueAlexandra Trkola
Apr 25, 2008·Current Protocols in Immunology·David C Montefiori
Jul 22, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Georgetta CannonDrew Weissman
Mar 5, 2009·Antimicrobial Agents and Chemotherapy·Carolina HerreraRobin J Shattock
Oct 7, 2009·American Journal of Respiratory Cell and Molecular Biology·Robert J KanerRonald G Crystal
Jan 22, 2010·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Jianhui NieYouchun Wang

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