PMID: 16518081Mar 7, 2006Paper

Effects of cell cycle status on the efficiency of liposome-mediated gene transfection in mouse fetal fibroblasts

The Journal of Reproduction and Development
Jian-Ning YuJing-He Tan

Abstract

Methods for cell cycle synchronization of mouse fetal fibroblast cells (MFFCs) were first selected and optimized. When MFFCs were cooled at 5 C for different periods of time, the highest percentage of cells at the G0/G1 phase (75.4+/-2.9%), with 3.5+/-0.3% of apoptotic cells, was achieved after 5 h of treatment. Extended cooling increased the number of apoptotic cells significantly. When MFFCs were treated with different concentrations of roscovitine (ROS) for different periods of time, the highest percentage of G0/G1 cells (83.5+/-1.8%), with 9.2+/-0.6% apoptotic cells, was obtained after exposure to 10 microM ROS for 24 h. When the cells were cooled at 5 C for 5 h followed by incubation in 10 microM ROS for 12 h, 83.6+/-1.9% were synchronized at the G0/G1 stage, with 3.6% undergoing apoptosis. Cell cycle progression was then observed after release of the MFFCs from different synchronization blocks. The highest percentages of S and G2/M cells (81% and 75%) were achieved at 12 and 20 h, respectively, after release of the MFFCs from the cooling plus ROS treatment, and these percentages were significantly higher than those obtained after release from the cooling or ROS alone blocks. Finally, MFFCs were transfected with pEGFP-N1 p...Continue Reading

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Citations

Jan 29, 2008·Zygote : the Biology of Gametes and Early Embryos·Zi-Li LeiDa-Yuan Chen
Nov 21, 2008·Molecular Human Reproduction·Jing-He TanJie Zhang
Feb 28, 2013·Expert Opinion on Drug Delivery·Abraham H Abouzeid, Vladimir P Torchilin
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Aug 4, 2015·Applied Biochemistry and Biotechnology·Sudhir Kumar JainBikash Chandra Sarkhel
Aug 4, 2006·Microscopy Research and Technique·Yong LiuJing-He Tan
Aug 12, 2020·Cellular Reprogramming·Érika Almeida PraxedesAlexsandra Fernandes Pereira
Apr 12, 2011·Biomedical Microdevices·Satoshi MigitaToshiyuki Ikoma
Jun 26, 2021·Advanced Healthcare Materials·Christina Cortez-JugoFrank Caruso

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis