Effects of cell storage and passage on basal and oxytocin-regulated prostaglandin secretion by equine endometrial epithelial and stromal cells

Theriogenology
A SzóstekD J Skarzynski

Abstract

Cell cultures are useful for determining the responses of specific cell types to various factors under controlled conditions and for obtaining a better understanding of in vivo physiologic processes. The aims of the present study were (i) to establish methodologies for isolation, culture and cryopreservation of equine endometrial epithelial and stromal cells; and (ii) to determine the effect of passage and cryopreservation on endometrial cell physiology, based on their basal and oxytocin (OT)-stimulated prostaglandin (PG) release. Epithelial and stromal cells were obtained by enzymatic digestion of equine endometrium collected from Days 2-5 of the estrous cycle (n = 16). Primary epithelial and stromal cells, as well as cryopreserved cells were stimulated with OT (10(-7)m) for 24 h. The concentrations of PGE(2) and PGF(2α) in the culture medium were measured by enzyme-linked immunosorbent assay (EIA). Oxytocin increased PGE(2) and PGF(2α) release by primary cultures of unfrozen epithelial cells until passage I (P < 0.01) and by the primary culture of unfrozen and cryopreserved/thawed stromal cells until passage IV (P < 0.01). Cryopreserved/thawed stromal cells cultured up to passage IV and unfrozen epithelial cells derived from ...Continue Reading

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Citations

Apr 11, 2014·Mediators of Inflammation·Anna Z SzóstekDariusz J Skarzynski
Feb 1, 2014·The Journal of Endocrinology·Anna Z SzóstekDariusz J Skarzynski
Feb 26, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Keisuke KozaiKiyoshi Okuda
Jan 24, 2017·Reproduction in Domestic Animals = Zuchthygiene·L LapkoH-A Schoon
Oct 13, 2017·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Rose SchwinghamerClaudia Klein
Oct 19, 2012·Reproduction, Fertility, and Development·A GalvãoG Ferreira-Dias
Jan 26, 2020·Scientific Reports·Anna Szóstek-MioduchowskaKiyoshi Okuda
May 23, 2020·Theriogenology·Anna Zuzanna Szóstek-MioduchowskaDariusz Jan Skarzynski
May 16, 2020·Animal Reproduction Science·Riley E ThompsonBudhan S Pukazhenthi

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