Effects of cell type and culture media on Interleukin-6 secretion in response to environmental particles

Toxicology in Vitro : an International Journal Published in Association with BIBRA
John M VeranthGarold S Yost

Abstract

Cultured lung cells provide an alternative to animal exposures for comparing the effects of different types of air pollution particles. Studies of particulate matter in vitro have reported proinflammatory cytokine signaling in response to many types of environmental particles, but there have been few studies comparing identical treatments in multiple cell types or identical cells with alternative cell culture protocols. We compared soil-derived, diesel, coal fly ash, titanium dioxide, and kaolin particles along with soluble vanadium and lipopolysaccharide, applied to airway-derived cells grown in submerged culture. Cell types included A549, BEAS-2B, RAW 264.7, and primary macrophages. The cell culture models (specific combinations of cell types and culture conditions) were reproducibly different in the cytokine signaling responses to the suite of treatments. Further, Interleukin-6 (IL-6) response to the treatments changed when the same cells, BEAS-2B, were grown in KGM versus LHC-9 media or in media containing bovine serum. The effect of changing media composition was reversible over multiple changes of media type. Other variables tested included culture well size and degree of confluence. The observation that sensitivity of a ...Continue Reading

References

Jul 3, 1998·American Journal of Respiratory Cell and Molecular Biology·M J Holtzman
Jul 3, 1998·American Journal of Respiratory Cell and Molecular Biology·J L QuayR B Devlin
Sep 5, 1998·The American Journal of Physiology·J M SametW Reed
Sep 9, 1998·American Journal of Respiratory Cell and Molecular Biology·T KennedyR B Devlin
Jun 11, 1999·The American Journal of Physiology·A J GhioJ M Samet
Nov 24, 1999·The American Journal of Physiology·M W FramptonR B Devlin
Jun 1, 2000·Journal of Toxicology and Environmental Health. Part a·R B HetlandP E Schwarze
Jul 7, 2000·Journal of Toxicology and Environmental Health. Part a·N GaoW E Wallace
Jun 21, 2001·Proceedings of the National Academy of Sciences of the United States of America·J R MastersP G Debenham
Feb 23, 2002·Toxicology and Applied Pharmacology·Bellina VeronesiMarga Oortgiesen
Jul 16, 2002·Environmental Health Perspectives·Ernesto Alfaro-MorenoAlvaro R Osornio-Vargas
Jul 18, 2002·Inhalation Toxicology·Ken Donaldson, C Lang Tran
Aug 9, 2002·Journal of Toxicology and Environmental Health. Part a·Haiyan XuBenoit Nemery
Oct 24, 2002·The European Respiratory Journal. Supplement·K F Chung
Jul 12, 2003·Journal of Toxicology and Environmental Health. Part a·JeanClare SeagraveSteven K Seilkop
Feb 6, 2004·Toxicology in Vitro : an International Journal Published in Association with BIBRA·R B HetlandE Dybing
Feb 6, 2004·Toxicology Letters·Laura E Pascal, Daniel M Tessier
Apr 20, 2004·Japanese Heart Journal·Tsugiyasu Kanda, Takashi Takahashi
Jul 29, 2004·Mutation Research·Marianne DybdahlHåkan Wallin
Aug 18, 2004·Toxicological Sciences : an Official Journal of the Society of Toxicology·John M VeranthGarold S Yost
Sep 4, 2004·Environmental Health Perspectives·Timothy R NurkiewiczMatthew A Boegehold
Aug 5, 2005·Environmental Health Perspectives·Susanne BeckerYuh-Chin T Huang
Aug 27, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Mark E JohansenGarold S Yost
Mar 2, 2006·Environmental Health Perspectives·John M VeranthGarold S Yost
Apr 18, 2006·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·K R Allen
May 16, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Gilman B AllenJason H T Bates
Jul 15, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Kevin R SmithKent E Pinkerton
Feb 16, 2007·Toxicological Sciences : an Official Journal of the Society of Toxicology·Christie M SayesDavid B Warheit

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Citations

Feb 8, 2014·Toxicology Letters·Laura CapassoMaurizio Gualtieri
Sep 12, 2009·Critical Reviews in Toxicology·Amy K Madl, Kent E Pinkerton
Oct 24, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Jorina GeysPeter H M Hoet
Sep 24, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Maurizio GualtieriMarina Camatini
Jun 16, 2010·Environmental Toxicology·Marina CamatiniMaurizio Gualtieri
Jun 1, 2010·Advanced Materials·Robert LandsiedelWendel Wohlleben
Feb 25, 2011·Particle and Fibre Toxicology·Alexandra KrollJürgen Schnekenburger
Dec 20, 2014·Journal of Applied Toxicology : JAT·Fei Zhao, Walter T Klimecki
Nov 12, 2013·Toxicology in Vitro : an International Journal Published in Association with BIBRA·T SkulandM Refsnes
Apr 30, 2013·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Carine DarollesVéronique Malard
Jun 25, 2010·Journal of Toxicology and Environmental Health. Part a·Song QuLung-Chi Chen
Jun 26, 2014·Experimental Biology and Medicine·Joan E NicholsJoaquin Cortiella
May 18, 2018·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·K C De GroveT Maes
Dec 14, 2011·Molecular Pharmacology·Cassandra E Deering-RiceChristopher A Reilly
Apr 28, 2021·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Hana BarosovaBarbara Rothen-Rutishauser

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