Effects of chain length and sulphur position of thia fatty acids on their incorporation into phospholipids in 7800 C1 hepatoma cells and isolated rat hepatocytes, and their effects on fatty acid composition of phospholipids

Biochemical Pharmacology
P WuJ Bremer

Abstract

Incorporation of thia fatty acids and their effects on the fatty acid composition in phospholipids has been investigated in 7800 C1 hepatoma cells and cultured hepatocytes. 3-Thia fatty acids of chain lengths from dodecyl-to hexadecyl-thioacetic acid were incorporated into phospholipids during a 3-day incubation. Longer and shorter 3-thia fatty acids were barely detectable. Tetradecylthioacetic acid, 3-thia stearate, and their delta9- desaturated derivatives were maximally incorporated into whole-cell phospholipids. The amount of tetradecylthioacetic acid incorporated into phospholipids of hepatoma cells remained almost identical in cells cultured for 3 days or adapted over a period of 1 year. Delta9-desaturated metabolites of long chain thia fatty acids (C13-to C16-S-acetic acid) were identified by GC-MS in phospholipids. 3-Thia stearate appeared to be the best substrated for delta9 desaturase. Incubation of hepatoma cells with thia fatty acids led to alterations in the amount of normal fatty acids in total phospholipids. The amounts of 16:0 and 18:1 decreased and 18:2 (n-6) and 20:5 (n-3) increased. Changes in the normal fatty acid composition of phospholipids were seen both with thia acids incorporated into phospholipids and...Continue Reading

References

Jul 1, 1977·Lipids·M E Hemler, W E Lands
Mar 1, 1991·Biochemical Pharmacology·E HvattumR K Berge
Apr 1, 1985·The Biochemical Journal·G M SmallM J Connock
Dec 7, 1995·Biochimica Et Biophysica Acta·U DiczfalusyS E Alexson

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Citations

Jan 20, 1998·Biochimica Et Biophysica Acta·K E HøvikJ Bremer
Jan 21, 1997·Biochimica Et Biophysica Acta·S SkredeJ Bremer
Jul 29, 2006·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·C Moya-FalcónB Ruyter

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