Effects of changes in extra- and intracellular K+ on the endothelial production of prostacyclin

British Journal of Pharmacology
J M Boeynaems, I Ramboer

Abstract

1. Changes in the KCl concentration of the incubation medium, from 0 to 80 mM, had no effect on the basal or ATP-stimulated release of prostacyclin (PGI2) from bovine aortic endothelial cells. 2. The monovalent cation ionophores, valinomycin and nigericin (5 microM), enhanced the release of PGI2 from endothelial cells stimulated by ATP or bradykinin. 3. The action of nigericin, unlike that of valinomycin, was time-dependent, abolished in a high-KCl medium and associated with an increased efflux of 86Rb and a time-dependent depletion of intracellular K+. 4. Ouabain (1-100 microM) also enhanced the release of PGI2 in response to ATP and induced a significant depletion of intracellular K+ in bovine aortic endothelial cells. 5. In conclusion, modifications of the endothelial cell membrane potential, resulting from changes in the extracellular K+ concentration, do not modulate the basal or ATP-stimulated production of PGI2. An acute depletion of intracellular K+ by nigericin or ouabain enhances the production of PGI2 in aortic endothelial cells stimulated by ATP or bradykinin.

References

Jan 1, 1976·Annual Review of Biochemistry·B C Pressman
Mar 15, 1977·Experimental Cell Research·M L Ledbetter, M Lubin
Jan 1, 1987·The Journal of Membrane Biology·H FichtnerM Kohlhardt
Jun 1, 1988·Circulation Research·S P OlesenD E Clapham
Dec 1, 1986·The Journal of Cell Biology·J M LarkinR G Anderson
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M Nakache, H E Gaub
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·A Lopez-RivasE Rozengurt
Jan 1, 1982·Methods in Enzymology·J Maclouf

❮ Previous
Next ❯

Citations

Dec 3, 2008·Journal of Physiology and Biochemistry·R Aras-LópezG Balfagón
Jan 1, 1990·Pharmacology & Therapeutics·R J Huxtable
Jan 1, 1990·Trends in Pharmacological Sciences·J M Boeynaems, J D Pearson
Sep 1, 1996·The Journal of Steroid Biochemistry and Molecular Biology·C Lobaccaro-HenriH Thaler-Dao
Jan 1, 1993·Journal of Cardiac Surgery·P ZillaM Deutsch
Aug 4, 2004·European Journal of Pharmacology·Pierre GuiraudouDino Nisato

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.