Effects of chlortetracycline and copper supplementation on the prevalence, distribution, and quantity of antimicrobial resistance genes in the fecal metagenome of weaned pigs

Preventive Veterinary Medicine
G E AggaMike D Tokach

Abstract

Use of in-feed antibiotics such as chlortetracycline (CTC) in food animals is fiercely debated as a cause of antimicrobial resistance in human pathogens; as a result, alternatives to antibiotics such as heavy metals have been proposed. We used a total community DNA approach to experimentally investigate the effects of CTC and copper supplementation on the presence and quantity of antimicrobial resistance elements in the gut microbial ecology of pigs. Total community DNA was extracted from 569 fecal samples collected weekly over a 6-week period from groups of 5 pigs housed in 32 pens that were randomized to receive either control, CTC, copper, or copper plus CTC regimens. Qualitative and quantitative PCR were used to detect the presence of 14 tetracycline resistance (tet) genes and to quantify gene copies of tetA, tetB, blaCMY-2 (a 3rd generation cephalosporin resistance gene), and pcoD (a copper resistance gene), respectively. The detection of tetA and tetB decreased over the subsequent sampling periods, whereas the prevalence of tetC and tetP increased. CTC and copper plus CTC supplementation increased both the prevalence and gene copy numbers of tetA, while decreasing both the prevalence and gene copies of tetB. In summary, t...Continue Reading

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Citations

Mar 10, 2016·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Getahun E AggaTerrance M Arthur
Sep 29, 2015·Canadian Journal of Microbiology·Devin B Holman, Martin R Chénier
Sep 8, 2015·Preventive Veterinary Medicine·Getahun E Agga, H Morgan Scott
Oct 11, 2017·Journal of the Science of Food and Agriculture·Katarzyna Skrypnik, Joanna Suliburska
Apr 12, 2020·Journal of Animal Science·Sandra Villagómez-EstradaDavid Solà-Oriol
Feb 13, 2021·Frontiers in Veterinary Science·Tara N GaireVictoriya V Volkova
May 28, 2021·Frontiers in Nutrition·Qi Su, Qin Liu
Nov 18, 2021·Journal of Applied Microbiology·Hui HanHongfu Zhang

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