PMID: 22568209May 10, 2012Paper

Effects of cholesterol depletion on the growth inhibition and apoptosis of human prostate cancer PC-3 cells

Zhonghua nan ke xue = National journal of andrology
Pei DongFang-Jian Zhou

Abstract

To investigate the regulatory effect of cholesterol depletion on the proliferation inhibition and apoptosis of human prostate cancer PC-3 cells. We cultured PC-3 cells in cholesterol depletion media (CDM) or normal media (NM), and added to the media platelet-derived growth factor (PDGF) and epidermal growth factor (EGF), respectively. Then we observed cell morphology under the inverse microscope, detected cell growth inhibition by MTT assay, and analyzed cell apoptosis and cycle phase by flow cytometry. After treated with CDM for 48 hours, the PC-3 cells obviously grew rounder and smaller, with more detached ones, and their proliferation remarkably inhibited in a dose-dependent manner, while their apoptosis rate not significantly increased, as compared with those treated with NM. After exposure to PDGF or EGF, the number of cells was increased in the NM group, but there were more detached and apoptotic cells in the CDM group than in the former (P < 0.05). Cell cycle analysis showed an increase in the number of G0/G1-phase cells and a decrease in that of S- and G2/M-phase cells in the CDM group compared with the NM group. Cholesterol depletion could significantly inhibit the growth of PC-3 cells without obvious induction of thei...Continue Reading

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

Ai zheng = Aizheng = Chinese journal of cancer
Qian-Yuan ZhuangZhang-Qun Ye
International Journal of Radiation Oncology, Biology, Physics
Shinji KariyaYasuhiro Ogawa
© 2021 Meta ULC. All rights reserved