Effects of cholesterol on physical properties of human erythrocyte membranes: impact on susceptibility to hydrolysis by secretory phospholipase A2

Biophysical Journal
Anne L HeinerJohn D Bell

Abstract

The ability of secretory phospholipase A(2) (sPLA(2)) to hydrolyze cell membranes is highly dependent on the physical properties of the membrane. The effects of cholesterol on these properties have been characterized in artificial bilayers and found to alter sPLA(2) activity significantly. It is hypothesized that the natural difference in cholesterol content between erythrocytes and leukocytes is in part responsible for their differing susceptibility to hydrolysis by sPLA(2). To test this hypothesis, defined amounts of cholesterol were removed from erythrocyte membranes using methyl-beta-cyclodextrin. Treatment of cells with methyl-beta-cyclodextrin increased the hydrolysis rate and total substrate hydrolyzed by sPLA(2). In general, this effect of cholesterol removal was more pronounced at higher temperatures. Comparison of the level of membrane order (assessed with the fluorescent probe laurdan) with hydrolysis rate revealed that sPLA(2) activity was greatly enhanced upon significant reductions in lipid order. Additional treatment of the cells with calcium ionophore further enhanced the hydrolysis rate and altered the relationship with membrane order. These data demonstrated that interactions with sPLA(2) observed in artificia...Continue Reading

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Citations

Mar 26, 2010·The Journal of Membrane Biology·María S JaureguiberryOmar J Rimoldi
Jan 30, 2014·Acta Biochimica Et Biophysica Sinica·María S JaureguiberryOmar J Rimoldi
Jan 8, 2016·Progress in Lipid Research·Mélanie CarquinDonatienne Tyteca
Mar 17, 2009·Biochimica Et Biophysica Acta·Christian CodePaavo K J Kinnunen
Nov 16, 2013·Plant Physiology·Patricia Gerbeau-PissotFrançoise Simon-Plas
Jun 4, 2021·Biological & Pharmaceutical Bulletin·Takeo YamaguchiShigemi Nagadome
Oct 16, 2008·Archives of Biochemistry and Biophysics·Alba A Vallejo, Marta S Fernández

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