PMID: 2484633Jul 1, 1989Paper

Effects of chromium(III) on DNA replication in vitro

Biological Trace Element Research
E T Snow, L S Xu

Abstract

A number of metal compounds are important environmental carcinogens; however, the molecular mechanisms of metal-induced genotoxicity are not yet understood. Chromium, for example, is substantially mutagenic in vivo and has been shown to decrease the DNA replication fidelity in vitro. But the mechanism of chromium-induced mutagenesis is unknown and the role of replication fidelity in chromium-induced carcinogenesis is unclear. We have used in vitro DNA replication assays to investigate the effects of chromium ions on DNA polymerase activity preliminary to studying their role in chromium-induced mutagenesis. Biologically active M13mp2 DNA was replicated with purified DNA polymerases in the presence of micromolar amounts of chromium with or without the normal divalent cation, magnesium. Nucleotide incorporation kinetics were determined and sequence specific pausing was analyzed by primer-extension. Our results have demonstrated an unexpected polymerase activation by low (0.5-5.0 microns) concentrations of chromium (III), although higher concentrations of chromium are increasingly inhibitory. The increased incorporation seem at low chromium(III) concentrations is the result of increased enzyme processivity and is not polymerase spe...Continue Reading

References

Aug 1, 1979·Chemico-biological Interactions·W D MacRaeH F Stich
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Jan 1, 1981·Toxicology·S K NiyogiD J Hoffman
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Citations

May 1, 1991·Environmental Health Perspectives·E T Snow
Jan 1, 1993·Critical Reviews in Toxicology·M D CohenM Costa
Apr 17, 2015·Environmental Health and Preventive Medicine·Arstan Abdramanovich MamyrbaevUmit Abulkhairovna Satybaldieva
Sep 1, 1994·Environmental Health Perspectives·E T Snow
Dec 19, 2012·Dalton Transactions : an International Journal of Inorganic Chemistry·V G VaidyanathanBalachandran Unni Nair
Apr 1, 2015·Asian Pacific Journal of Cancer Prevention : APJCP·Yerbol BekmukhambetovArstan Mamyrbayev
Jul 17, 2020·Carcinogenesis·Yusha Zhu, Max Costa
Nov 12, 2005·Molecular and Cellular Biochemistry·Jamie L FornsaglioSteven R Patierno
Oct 8, 2009·Journal of Basic Microbiology·Borut PoljsakMiklós Pesti
Jan 1, 1992·Pharmacology & Therapeutics·E T Snow
Mar 1, 1990·Mutation Research·S De FloraP Zanacchi
Sep 1, 1993·Chemico-biological Interactions·E T SnowP L Kinney
Apr 1, 1990·Mutation Research·C A Miller, M Costa

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