PMID: 7007871Dec 16, 1980Paper

Effects of chronic exposure to insulin on lipid synthesis in 3T3-L1 fatty fibroblasts

Molecular and Cellular Biochemistry
M S Martin, S L Pohl

Abstract

The acute effects of insulin on 3H incorporation into lipid from glucose was measured in 3T3-L1 fatty fibroblasts cultured with and without insulin at 10 microgram/ml for 7 days. Basal lipid synthesis did not differ between control cells and cells treated chronically with insulin. There was no insulin stimulation in treated cells while 3H incorporation into lipid in control cells increased from a basal level of 1.39 to 3.85 nmol/dish/90 min with a maximally-stimulating concentration of insulin. This is the first study of 3T3-L1 fatty fibroblasts which describes a lack of acute insulin responsiveness in cells exposed chronically to insulin as compared to control cells.

References

Dec 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·T R Russell, R Ho
Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·B C ReedM D Lane
Jun 1, 1978·Nature·J N LivingstonD H Lockwood
Jan 1, 1974·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·A J MoodyJ Gliemann
Oct 1, 1980·The Journal of Clinical Endocrinology and Metabolism·J StevensS L Pohl

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Citations

Jan 1, 1984·Metabolism: Clinical and Experimental·L Olansky, S L Pohl
Jan 1, 1989·Developmental Genetics·S L MarbanJ D Gearhart
Aug 1, 1990·Diabetes Research and Clinical Practice·M J SissionsJ Best

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