Effects of chronic hypokalemia on renal expression of the "gastric" H(+)-K(+)-ATPase alpha-subunit gene

The American Journal of Physiology
K Y AhnB C Kone

Abstract

Chronic potassium restriction leads to active potassium reabsorption in the late distal nephron and collecting duct, segments known to express "gastric" H(+)-K(+)-adenosinetriphosphatase (H(+)-K(+)-ATPase) alpha-subunit mRNA. In this study, the cellular distribution and relative abundance of mRNA encoding this isoform were examined in kidneys of normal and potassium-deprived (2 wk) rats. In situ hybridization with isoform-specific cRNA probes demonstrated prominent expression of this gene in the connecting segment (CNT), entire collecting duct, and renal papillary surface epithelium in a comparable distribution in both groups of rats. Hypertrophy of the outer medullary collecting ducts in the inner stripe of potassium-restricted rats was observed. Competitive polymerase chain reaction analysis revealed twofold greater levels of gastric H(+)-K(+)-ATPase alpha-subunit mRNA (normalized to the level of beta-actin mRNA) in the cortex, but roughly comparable levels in the outer and inner medulla, of potassium-restricted rats compared with controls. These data suggest that chronic potassium restriction results in modestly enhanced renal cortical expression of the gastric H(+)-K(+)-ATPase alpha-subunit gene and that this isoform may pa...Continue Reading

Citations

Jan 12, 2001·Physiological Reviews·S Muto
Jul 18, 2001·American Journal of Physiology. Renal Physiology·G Fejes-Tóth, A Náray-Fejes-Tóth
Dec 12, 2012·American Journal of Physiology. Renal Physiology·Jesse M BishopI David Weiner
Mar 7, 2019·American Journal of Physiology. Renal Physiology·Viktor TomilinOleh Pochynyuk
Dec 4, 2019·Physiological Reviews·Ewout J HoornRobert A Fenton
Sep 13, 2001·American Journal of Physiology. Renal Physiology·J A KrautG Sachs
Jul 31, 2009·American Journal of Physiology. Renal Physiology·Michelle L GumzCharles S Wingo
Nov 30, 2000·American Journal of Physiology. Renal Physiology·N Laroche-JoubertA Doucet

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