Effects of chronic REM sleep restriction on D1 receptor and related signal pathways in rat prefrontal cortex

BioMed Research International
Yan HanWenling Ma

Abstract

The prefrontal cortex (PFC) mediates cognitive function that is sensitive to disruption by sleep loss, and molecular mechanisms regulating neural dysfunction induced by chronic sleep restriction (CSR), particularly in the PFC, have yet to be completely understood. The aim of the present study was to investigate the effect of chronic REM sleep restriction (REM-CSR) on the D1 receptor (D1R) and key molecules in D1R' signal pathways in PFC. We employed the modified multiple platform method to create the REM-CSR rat model. The ultrastructure of PFC was observed by electron microscopy. HPLC was performed to measure the DA level in PFC. The expressions of genes and proteins of related molecules were assayed by real-time PCR and Western blot, respectively. The general state and morphology of PFC in rats were changed by CSR, and DA level and the expression of D1R in PFC were markedly decreased (P < 0.01, P < 0.05); the expression of phosphor-PKAcα was significantly lowered in CSR rats (P < 0.05). The present results suggested that the alteration of neuropathology and D1R expression in PFC may be associated with CSR induced cognitive dysfunction, and the PKA pathway of D1R may play an important role in the impairment of advanced neural ...Continue Reading

References

Sep 24, 2004·Progress in Neurobiology·Jeremy K Seamans, Charles R Yang
May 3, 2005·Behavioural Brain Research·Ricardo Borges MachadoSergio Tufik
Mar 10, 2007·Cellular and Molecular Life Sciences : CMLS·T W BoonstraP J Beek
Mar 7, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Verena Pawlak, Jason N D Kerr
Sep 2, 2009·Anais Da Academia Brasileira De Ciências·Sergio TufikMarco T de Mello
May 6, 2010·International Journal of Occupational Medicine and Environmental Health·Jolanta Orzeł-Gryglewska
Jun 16, 2010·Pharmacology & Therapeutics·Ashiwel S Undieh
Nov 26, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Melissa R Warden, Earl K Miller
Dec 17, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Aaron J GruberPatricio O'Donnell
Aug 31, 2011·Neurobiology of Learning and Memory·John G McCoy, Robert E Strecker
Oct 4, 2011·Brain Research·Bradley D WintersJames M Krueger
Mar 26, 2013·Current Opinion in Neurobiology·Mathias BasnerDavid F Dinges
May 2, 2014·Frontiers in Neural Circuits·Kelsey L Clark, Behrad Noudoost
May 6, 2014·Neuroscience and Biobehavioral Reviews·Matthew L Dixon, Kalina Christoff

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
PCRs
electrophoresis

Software Mentioned

GraphPad Prism
GraphPad

Related Concepts

Related Feeds

Advanced Imaging of Cellular Signaling

Cell signaling is a vital mechanism for communication within cells and outside with the environment. Several different signaling pathways have been found and advanced imaging techniques are being developed to visualize the molecules involved in these signaling pathways. Find the latest research in advanced imaging of cellular signaling here.