Effects of cis-platinum(II) diamminedichloride on survival and the rate of DNA synthesis in synchronously growing Chinese hamster V79-379A cells in the absence and presence of caffeine inhibited post-replication repair; evidence for an inducible repair mechanism

Chemico-biological Interactions
H N Fraval, J J Roberts

Abstract

The mechanism by which cis-Platinum(II)diamminedichloride, (cis-Pt(II)), inhibited DNA synthesis was investigated using synchronously growing Chinese hamster V79-379A cells. The progression of G1 phase treated cells into S phase was not inhibited. However the rate of DNA synthesis throughout the S phase was depressed in a dose dependent manner compared to a control. The peak rate of DNA synthesis was delayed and the duration of the S phase was extended resulting in a dose dependent delay in the timing of mitosis in the first cell cycle after treatment. The delay in the timing of mitosis after the second S phase in treated cells was comparable to the delay observed after the first cycle. Post treatment incubation of cells in the presence of a non toxic concentration of caffeine resulted in an alteration in the pattern of DNA synthesis, leading to a reversal of the dose dependent delay in the timing of the peak rate of DNA synthesis to a position corresponding to the control. Caffeine potentiated the toxicity of cis-Pt(II) only if it was added during the first cell cycle after treatment. The patterns of DNA synthesis in synchronous cultures of Chinese hamster V79-379A cells after cis-Pt(II) treatment appeared to be similar to tho...Continue Reading

References

Feb 15, 1978·Biochemical Pharmacology·M J Tisdale, J J Roberts
Mar 7, 1973·Journal of the American Chemical Society·S MansyA J Thomson

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Citations

Aug 1, 1981·Quarterly Reviews of Biophysics·R MeneghiniR I Schumacher
Mar 2, 2011·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Yoshikazu TanzawaKatsuro Tomita
Feb 1, 1992·Japanese Journal of Cancer Research : Gann·T Tashiro, Y Sato
Jan 1, 1985·The British Journal of Radiology·H A BarotA H Nias
Sep 1, 1985·Experimental Cell Research·M KapiszewskaD Wlodek
Jan 1, 1988·Experimental Brain Research·E A KeshnerB Debu
Oct 24, 2015·Nature Reviews. Immunology·Gregory W Goldberg, Luciano A Marraffini
Nov 1, 1986·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·A C BeggH Bartelink
Jan 2, 2003·The Journal of Biological Chemistry·Aleksandra MandicMaria C Shoshan
Jan 1, 1991·Medical Oncology and Tumor Pharmacotherapy·R HenrikssonK Grankvist

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