Effects of CK2 inhibition in cultured fibroblasts from Type 1 Diabetic patients with or without nephropathy

Growth Factors
Elisabetta IoriPaolo Tessari

Abstract

CK2 is a multifunctional, pleiotropic protein kinase involved in the regulation of cell proliferation and survival. Since fibroblasts from Type 1 Diabetes patients (T1DM) with Nephropathy exhibit increased proliferation, we studied cell viability, basal CK2 expression and activity, and response to specific CK2 inhibitors TBB (4,5,6,7-tetrabenzotriazole) and CX4945, in fibroblasts from T1DM patients either with (T1DM+) or without (T1DM-) Nephropathy, and from healthy controls (N). We tested expression and phosphorylation of CK2-specific molecular targets. In untreated fibroblasts from T1DM+, the cell viability was higher than in both N and T1DM-. CK2 inhibitors significantly reduced cell viability in all groups, but more promptly and with a larger effect in T1DM+. Differences in CK2-dependent phosphorylation sites were detected. In conclusion, our results unveil a higher dependence of T1DM+ cells on CK2 for their survival, despite a similar expression and a lower activity of this kinase compared with those of normal cells.

References

Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J SommercornE G Krebs
Dec 12, 1996·Biochimica Et Biophysica Acta·L A Pinna, M Ruzzene
Feb 15, 1997·Archives of Biochemistry and Biophysics·S Zhang, K H Kim
Feb 20, 1998·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·D K JinA J Fish
Sep 21, 2000·Kidney International. Supplement·G Wolf
Sep 24, 2002·Journal of Cell Science·Lorenzo A Pinna
Mar 13, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Flavio Meggio, Lorenzo A Pinna
Apr 9, 2005·Cell Death and Differentiation·G Di MairaM Ruzzene
May 18, 2005·Proceedings of the National Academy of Sciences of the United States of America·Masateru YamadaGozoh Tsujimoto
Aug 23, 2005·Journal of Diabetes and Its Complications·Anna MaestroniGianpaolo Zerbini
Apr 15, 2006·Circulation Research·Ingrid Fleming
Jul 11, 2006·The Journal of Clinical Endocrinology and Metabolism·Lucia PuricelliPaolo Tessari
Jul 3, 2007·Cell·Brendan D Manning, Lewis C Cantley
May 22, 2008·Advances in Enzyme Regulation·Kashif A AhmadKhalil Ahmed
Jul 1, 2008·The Biochemical Journal·Mario A PaganoLorenzo A Pinna
Apr 24, 2009·Cellular and Molecular Life Sciences : CMLS·Y Miyata
Jun 16, 2009·Medicinal Research Reviews·Giorgio CozzaStefano Moro
Aug 12, 2009·Biochimica Et Biophysica Acta·Maria Ruzzene, Lorenzo A Pinna
Mar 27, 2010·Cellular and Molecular Life Sciences : CMLS·Rui MengMathias Montenarh
Jun 10, 2010·BioFactors·Janeen H TrembleyKhalil Ahmed
Jun 26, 2010·Medicinal Research Reviews·Daniel I PerezAna Martinez
Sep 21, 2010·Biochemical and Biophysical Research Communications·Rui MengMathias Montenarh
Nov 3, 2010·Methods in Enzymology·Maria RuzzeneLorenzo A Pinna
Oct 16, 2013·The International Journal of Biochemistry & Cell Biology·Sabrina WelkerMathias Montenarh

❮ Previous
Next ❯

Citations

Feb 5, 2016·Advances in Hematology·Alessandro MorottiGiuseppe Saglio
Jul 8, 2020·International Journal of Molecular Sciences·Rebecca ScheuerClaudia Götz
May 18, 2021·Signal Transduction and Targeted Therapy·Christian BorgoMaria Ruzzene

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.