Effects of CO2 and pH on the spinal respiratory rhythm generator in vitro

Brain Research Bulletin
D Dubayle, Denise Viala

Abstract

In vitro brainstem spinal cord preparations isolated from newborn rats were used to separately test the effects of modifications of FCO2 and pH of artificial cerebrospinal fluid on the frequency and amplitude of spinal respiratory activity recorded from C2-C8 ventral roots. Different substances such as L-glutamic acid (3 x 10[-3] M), N-methyl-D-aspartic acid (5 x 5 x 10[-6] M), amphetamine (6 mg/100 ml), 5-hydroxytryptophane (10[-3] M), or modified K+ (10[-3] M) were tested for their capacity to elicit stable changes in spinal respiratory activity over a long time period (more than 30 min) and with high frequency of occurrence, i.e., in at least 50% of the cases. None of the above drugs were found to be suitable for the investigation of the chemosensitivity of the spinal respiratory generator (sRG) because they were only able to maintain spinal respiratory activity for around 15 min. Given these data, the previously used procedure of activation through initial deep diethyl ether anaesthesia of newborn rats was employed [3] to test the chemosensitivity of the sRG because this treatment resulted in the maintenance of spinal respiratory activity with a regular pattern for 30 min, even if it occurred in only 25% of the preparations...Continue Reading

References

Dec 1, 1992·Current Opinion in Neurobiology·D W RichterS Schwarzacher

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Citations

Apr 27, 2010·The Journal of Physiological Sciences : JPS·Suguru KobayashiMamoru Aoki
Sep 17, 2019·The Journal of Spinal Cord Medicine·Michael George Zaki Ghali
Apr 5, 2020·Neural Regeneration Research·George Zaki GhaliEmil Zaki Ghali
Aug 5, 2015·Journal of Neurotrauma·Michael George Zaki Ghali, Vitaliy Marchenko

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