Effects of coagulation factor XIII on intestinal functional capillary density, leukocyte adherence and mesenteric plasma extravasation in experimental endotoxemia

Critical Care : the Official Journal of the Critical Care Forum
J BirnbaumChristian Lehmann

Abstract

The objective of this study was to determine the effects of the administration of the coagulation factor XIII (F XIII) on intestinal functional capillary density, leukocyte adherence and mesenteric plasma extravasation during experimental endotoxemia. In a prospective, randomized, controlled animal study 42 male Wistar rats were divided into three groups. Group 1 served as the control group. Groups 2 (lipopolysaccharide (LPS) group) and 3 (F XIII group) received endotoxin infusions (2.5 mg/kg/h for 2 hours). In group 3, 50 U/kg body weight F XIII was continuously administered during the first 30 minutes of endotoxemia. F XIII levels were measured in all animals. One half of the animals of each group were studied for intestinal functional capillary density (FCD) and leukocyte adherence on venular endothelium by intravital fluorescence microscopy (IVM). In the other half of each group, mesenteric plasma extravasation (FITC-albumin) was determined by IVM. The F XIII level was significantly increased in the F XIII treatment group. In the LPS group, endotoxemia led to a significant reduction of mucosal FCD (-18.5%; p < 0.01 versus control group). F XIII administration in the F XIII group attenuated the decrease in mucosal FCD (-3.7%...Continue Reading

References

Nov 1, 1977·Microvascular Research·G W Schmid-SchoenbeinS Kovalcheck
Jan 1, 1991·Annals of the New York Academy of Sciences·D F MosherE L Barry
Jan 1, 1982·Intensive Care Medicine·J WitteH Fritz
Feb 18, 1995·Lancet·R LorenzM Classen
May 4, 1999·The Journal of Experimental Medicine·T NollH M Piper
Jun 24, 1999·Thrombosis Research·L MuszbekZ Hevessy
Jul 23, 1999·International Journal of Oral and Maxillofacial Surgery·I E el-Hakim
Jul 4, 2001·Digestion·G D'ArgenioG Mazzacca
Aug 24, 2002·Hämostaseologie·Th Noll, G Wozniak
Aug 24, 2002·Hämostaseologie·G Wozniak, Th Noll
Mar 29, 2003·Intensive Care Medicine·Mitchell M LevyUNKNOWN International Sepsis Definitions Conference
Jun 14, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Rima DardikAida Inbal
Jul 16, 2003·Cellular and Molecular Life Sciences : CMLS·R Adány, H Bárdos
May 28, 2004·The British Journal of Surgery·N CollighanM C Bellamy

❮ Previous
Next ❯

Citations

Jul 9, 2009·Blood·Mieke Delvaeye, Edward M Conway
May 29, 2013·Critical Care : the Official Journal of the Critical Care Forum·Su Jin JeongJune Myung Kim
Sep 4, 2007·Critical Care : the Official Journal of the Critical Care Forum·Vladislava SimkovaEnrico Calzia
Apr 25, 2009·Current Opinion in Anaesthesiology·Daniel De BackerRaphaël Favory
Feb 6, 2015·Thrombosis and Haemostasis·Gerhard DickneiteMarco Matucci Cerinic
Mar 4, 2008·Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie·Christian LehmannDragan Pavlovic
Jan 3, 2013·Journal of Thrombosis and Haemostasis : JTH·V Schroeder, H P Kohler
May 23, 2019·Frontiers in Immunology·Felix HaussnerMarkus Huber-Lang
Apr 21, 2010·Best Practice & Research. Clinical Anaesthesiology·Wolfgang Korte

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence microscopy

Software Mentioned

SigmaStat

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.